PXD013265

PXD013265 is an original dataset announced via ProteomeXchange.

Title	Integration of metabolomics and transcriptomics to reveal metabolic characteristics and key targets associated with cisplatin resistance in non-small cell lung cancer
Description	Continuous exposure to cisplatin can induce drug resistance to limit efficacy, however, the underlying mechanisms correlated to cisplatin resistance are still unclear. Drug-sensitive A549 cells and cisplatin-resistant A549/DDP cells were used to explore the potential metabolic pathways and key targets associated with cisplatin resistance by integrating untargeted metabolomics with transcriptomics. The results of comprehensive analyses showed that 19 metabolites were significantly changed in A549/DDP vs A549 cells, and some pathways had a close relationship with cisplatin resistance, such as the biosynthesis of aminoacyl-tRNA, glycerophospholipid metabolism, and glutathione metabolism. Moreover, transcriptomics analysis showed glutathione metabolism was also obviously affected in A549/DDP, which indicated that glutathione metabolism played an import role in the process of drug resistance. Meanwhile, transcriptomics analysis suggested the four enzymes related to glutathione metabolism - CD13, GPX4, RRM2B, and OPLAH - as potential targets of cisplatin resistance in NSCLC. Further studies identified the over-expressions of these four enzymes in A549/DDP. The elucidation of mechanism and discovery of new potential targets may help us have a better understanding of cisplatin resistance.
HostingRepository	PRIDE
AnnounceDate	2019-08-12
AnnouncementXML	Submission_2019-08-12_05:20:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	shi yuhuan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Agilent MassHunter format

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-27 03:08:49	ID requested
⏵ 1	2019-08-12 05:20:28	announced

Shi Y, Wang Y, Huang W, Wang Y, Wang R, Yuan Y, Integration of Metabolomics and Transcriptomics To Reveal Metabolic Characteristics and Key Targets Associated with Cisplatin Resistance in Nonsmall Cell Lung Cancer. J Proteome Res, 18(9):3259-3267(2019) [pubmed]

submitter keyword: metabolomics;transcriptomics;cisplatin resistance

NSCLC

Yongfang Yuan1
contact affiliation	Department of Pharmacy, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine
contact email	nmxyyf@126.com
lab head
shi yuhuan
contact affiliation	1. Department of Pharmacy, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine
contact email	syh1195463038@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013265

PRIDE project URI

• PRIDE
  1. PXD013265
     1. Label: PRIDE project
     2. Name: Integration of metabolomics and transcriptomics to reveal metabolic characteristics and key targets associated with cisplatin resistance in non-small cell lung cancer