PXD013257

PXD013257 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of pepper (Capsicum annuum L.) seedlings under selenium stress
Description	Selenium (Se) is an essential trace element for human and animal health. Se fertilizer has been used to increase the Se content in crops to meet the Se requirements in humans and animals. To address the challenge of Se poisoning in plants, the mechanisms underlying Se-induced stress in plants must be understood. Here, to elucidate the effects of Se stress on the protein levels in pepper, we used an integrated approach involving tandem mass tag labeling, HPLC fractionation, and mass spectrometry-based analysis. A total of 4,693 proteins were identified, 3,938 of which yielded quantitative information. Among them, the expression of 172 proteins was up-regulated, and the expression of 28 proteins was down-regulated in the Se/mock treatment comparison. According to the above data, we performed a systematic bioinformatics analysis of all identified proteins and differentially expressed proteins (DEPs). The DEPs were most strongly associated with the terms ‘metabolic process’, ‘posttranslational modification, protein turnover, chaperones,’ and ‘protein processing in endoplasmic reticulum’ according to GO, KOG classification, and KEGG enrichment analysis, respectively. Furthermore, several heat shock proteins were identified as DEPs. These results provide insights that may facilitate further studies on the pepper proteome expressed downstream of the Se stress response. Our data revealed that the responses of pepper to Se stress involve various pathways.
HostingRepository	PRIDE
AnnounceDate	2019-09-16
AnnouncementXML	Submission_2019-09-16_01:14:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaonan fu
SpeciesList	scientific name: Capsicum annuum; NCBI TaxID: 4072;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-26 09:47:05	ID requested
⏵ 1	2019-09-16 01:14:36	announced

Dataset with its publication pending

submitter keyword: seedlings,proteomic analysis,selenium stress,Capsicum annuum L.

Chenghao Zhang
contact affiliation	Institute of Agricultural Equipment, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
contact email	sheshizaopei@163.com
lab head
xiaonan fu
contact affiliation	zhejiang scientific technology universitiy
contact email	2016460649@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013257
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of pepper (Capsicum annuum L.) seedlings under selenium stress