PXD013256

PXD013256 is an original dataset announced via ProteomeXchange.

Title	Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes
Description	Transcription initiation involves the coordinated activities of large multimeric complexes that are organized into functional modules. Little is known about the mechanisms and pathways that govern their assembly from individual components. We report here several principles governing the assembly of the highly conserved SAGA and NuA4 co-activator complexes. Using fission yeast, which contain two functionally non-redundant paralogs of the shared Tra1 subunit, we demonstrate that Tra1 contributes to scaffolding the entire NuA4 complex. In contrast, within SAGA, Tra1 specifically promotes the incorporation of the de-ubiquitination module (DUB), defining an ordered assembly pathway. Biochemical and functional analyses elucidated the mechanism by which Tra1 assemble differentially into SAGA or NuA4 and identified a small, conserved region of Spt20 that is both necessary and sufficient to anchor Tra1 within SAGA. Finally, we establish that Hsp90 and its cochaperone TTT are required for Tra1 de novo incorporation into both SAGA and NuA4, indicating that Tra1, a pseudokinase of the PIKK family, shares a dedicated chaperone machinery with its cognate kinases. Overall, our work brings mechanistic insights into the de novo assembly of transcriptional complexes through ordered pathways and reveals the contribution of dedicated chaperones to this process.
HostingRepository	PRIDE
AnnounceDate	2019-10-02
AnnouncementXML	Submission_2019-11-25_00:42:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oana Vigy
SpeciesList	scientific name: Schizosaccharomyces pombe 972h-; NCBI TaxID: 284812;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-26 09:30:30	ID requested
1	2019-10-02 08:13:12	announced
⏵ 2	2019-11-25 00:42:34	announced	2019-11-25: Updated publication reference for PubMed record(s): 31748520.

El, í, as-Villalobos A, Toullec D, Faux C, S, é, veno M, Helmlinger D, Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in yeast. Nat Commun, 10(1):5237(2019) [pubmed]

submitter keyword: Transcription, Chromatin, Multimeric complex, Chaperone

Dominique Helmlinger
contact affiliation	Regulation of gene expression, CRBM, France
contact email	dhelmlinger@crbm.cnrs.fr
lab head
Oana Vigy
contact affiliation	FPP CNRS
contact email	oana.vigy@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD013256

PRIDE project URI

• PRIDE
  1. PXD013256
     1. Label: PRIDE project
     2. Name: Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes