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PXD013251

PXD013251 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHomeostatic and Interferon-induced gene expression represent different states of promoter-associated transcription factor ISGF3
DescriptionHost defense by the innate immune system requires the establishment of antimicrobial states allowing cells to cope with microorganisms before the onset of the adaptive immune response. Interferons (IFN) are of vital importance in the establishment of cell-autonomous antimicrobial immunity. Speed is therefore an important attribute of the cellular response to IFN. With much of the antimicrobial response being installed de novo, this pertains foremost to gene expression, the rapid switch between resting-state and active-state transcription of host defense genes. Mechanisms to meet this demand on the relevant molecular machinery include remodeling of chromatin but also changes in transcription factor interaction prior and during the IFN response. Our results show how distinct transcription factor complexes, determine the responsiveness of Interferon stimulated genes to different IRF9-containing complexes. Raw 264.7 macrophages expressing a doxycycline-inducible, myc-tagged versions of each IRF9-BirA*, STAT2-BirA* and STAT1-BirA* fusion genes were used to study complex formation in vivo. Furthermore, we extended the BioID proximity labeling by coupling it to parallel reaction monitoring to determine the degree and quantity of association between IRF9 and STATs in resting and interferon-treated macrophages.
HostingRepositoryPanoramaPublic
AnnounceDate2019-07-15
AnnouncementXMLSubmission_2019-07-15_09:22:26.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterThomas Gossenreiter
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListCarbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-26 08:15:38ID requested
12019-07-15 09:22:27announced
Publication List
Platanitis E, Demiroz D, Schneller A, Fischer K, Capelle C, Hartl M, Gossenreiter T, Müller M, Novatchkova M, Decker T, A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription. Nat Commun, 10(1):2921(2019) [pubmed]
Keyword List
submitter keyword: Targeted MS, PRM, quantification, BioID
Contact List
Thomas Decker
contact affiliationMax F. Perutz Laboratories (MFPL), University of Vienna, Austria
contact emailthomas.decker@univie.ac.at
lab head
Thomas Gossenreiter
contact affiliationMass Spectrometry Facility, Max F. Perutz Laboratories (MFPL), Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, A-1030 Vienna, Austria
contact emailthomas.gossenreiter@univie.ac.at
dataset submitter
Full Dataset Link List
Panorama Public dataset URI