PXD013250

PXD013250 is an original dataset announced via ProteomeXchange.

Title	Proteomic profile of extracellular matrix-based scaffold obtained by culturing a murine spleen stromal cell line
Description	Lymph nodes are secondary lymphoid tissues that play a critical role in filtering the lymph and supporting adaptive immune responses. Surgical resection of LNs, radiation therapy or infections may damage lymphatic vasculature and compromised immune functions. Here, we describe the generation of functional synthetic lympho-organoids (LOs) using LN stromal progenitors and decellularized extra cellular matrix-based scaffolds. We show that upon transplantation at the site of resected LNs, LOs become integrated into the endogenous lymphatic vasculature and efficiently restore lymphatic drainage and perfusion. Upon immunization, LOs support the activation of antigen-specific immune responses, thus acquiring properties of native lymphoid tissues. These findings provide the first proof-of-concept for the development of synthetic lympho-organoids suitable to restore lymphatic and immune cell functions.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-11_18:14:58.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013250
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Annapaola Andolfo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-26 08:10:10	ID requested
⏵ 1	2019-11-11 18:15:00	announced

Lenti E, Bianchessi S, Proulx ST, Palano MT, Genovese L, Raccosta L, Spinelli A, Drago D, Andolfo A, Alfano M, Petrova TV, Mukenge S, Russo V, Brendolan A, Therapeutic Regeneration of Lymphatic and Immune Cell Functions upon Lympho-organoid Transplantation. Stem Cell Reports, 12(6):1260-1268(2019) [pubmed]

curator keyword: Biological

submitter keyword: Extracellular matrix, lympho-organoids, transplantation, mouse

Andrea Brendolan
contact affiliation	Laboratory of Lymphoid Organ Development Division of Experimental Oncology San raffaele Scientific Institute Via Olgettina, 60 20132 Milan (Italy)
contact email	brendolan.andrea@hsr.it
lab head
Annapaola Andolfo
contact affiliation	Ospedale San Raffaele
contact email	andolfo.annapaola@hsr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013250

PRIDE project URI

• PRIDE
  1. PXD013250
     1. Label: PRIDE project
     2. Name: Proteomic profile of extracellular matrix-based scaffold obtained by culturing a murine spleen stromal cell line