PXD013243

PXD013243 is an original dataset announced via ProteomeXchange.

Title	Morphological plasticity in a sulfur-oxidizing bacterium from the SUP05 clade enhances dark carbon fixation
Description	Sulfur-oxidizing bacteria from the SUP05 clade are abundant in anoxic and oxygenated marine waters that appear to lack reduced sources of sulfur for cell growth. This raises questions about how these chemosynthetic bacteria survive across oxygen and sulfur gradients and how their mode of survival impacts the environment. Here we use growth experiments, proteomics and cryo-electron tomography to show that a SUP05 isolate, Ca. Thioglobus autotrophicus, is several times larger, amorphous in shape and stores considerably more intracellular sulfur when it respires oxygen. We also show that these cells can use diverse sources of reduced organic and inorganic sulfur at submicromolar concentrations. Enhanced cell size, carbon content and metabolic activity of the aerobic phenotype are likely facilitated by a stabilizing surface-layer (S-layer) and an uncharacterized form of FtsZ-less cell division that supports morphological plasticity. The additional sulfur storage provides an energy source that allows cells to continue metabolic activity when exogenous sulfur sources are not available. This metabolic flexibility leads to the production of more organic carbon in the ocean than estimates that are based solely on their anaerobic phenotype.
HostingRepository	PRIDE
AnnounceDate	2019-05-22
AnnouncementXML	Submission_2019-05-22_00:32:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Lundeen
SpeciesList	scientific name: Candidatus Thioglobus autotrophicus; NCBI TaxID: 1705394;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-26 07:30:05	ID requested
⏵ 1	2019-05-22 00:32:05	announced

Shah V, Zhao X, Lundeen RA, Ingalls AE, Nicastro D, Morris RM, Morphological Plasticity in a Sulfur-Oxidizing Marine Bacterium from the SUP05 Clade Enhances Dark Carbon Fixation. mBio, 10(3):(2019) [pubmed]

submitter keyword: SUP05, Gammaproteobacteria, QExactive HF, Shotgun Proteomics

Anitra Ingalls
contact affiliation	School of Oceanography, University of Washington
contact email	aingalls@uw.edu
lab head
Rachel Lundeen
contact affiliation	University of Washington
contact email	ralundeen@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013243

PRIDE project URI

• PRIDE
  1. PXD013243
     1. Label: PRIDE project
     2. Name: Morphological plasticity in a sulfur-oxidizing bacterium from the SUP05 clade enhances dark carbon fixation