PXD013234

PXD013234 is an original dataset announced via ProteomeXchange.

Title	Comparison of proteome composition of serum enriched in extracellular vesicles isolated from polycythemia vera patients and healthy controls
Description	Extracellular vesicles (EVs), e.g., exosomes and microvesicles, are one of the main networks of intercellular communication. In myeloproliferative disorders, such as polycythemia vera (PV), excess of EVs originating from overabundant blood cells can directly contribute to thrombosis through their procoagulant activity. However, proteomic composition of these vesicles in PV patients has not been investigated before. In this work, we examined proteomic composition of serum exosomes of PV patients in comparison to healthy controls. We processed exosome-enriched serum samples using Multiple Enzyme Filter Aided Sample Preparation approach (MED-FASP), conducted LC-MS/MS measurements on a Q-Exactive HF-X mass spectrometer, and quantitatively analyzed the absolute concentrations of identified proteins by Total Protein Approach (TPA). 38 proteins were present at statistically significant different concentrations between PV patients’ study group and healthy controls’ group. The main protein components deregulated in PV were primarily related with excessive amounts of cells (TFRC, SELL, GP5), increased platelet activation (SERPINE1, MMRN1), elevated immune and inflammatory response (HPSE, CAMP, LYZ, SELL, LTF), and high concentrations of procoagulant and angiogenetic agents (ANG, HPSE). Our study provides the first quantitative analysis of the serum exosomes’ proteome in PV patients. Acquired knowledge can be beneficial to understanding of the mechanism of PV disease and further development of diagnostic or therapeutic procedures.
HostingRepository	PRIDE
AnnounceDate	2019-05-13
AnnouncementXML	Submission_2019-05-13_00:48:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacek Wisniewski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-26 05:43:25	ID requested
⏵ 1	2019-05-13 00:48:30	announced

Fel A, Lewandowska AE, Petrides PE, Wi, ś, niewski JR, Comparison of Proteome Composition of Serum Enriched in Extracellular Vesicles Isolated from Polycythemia Vera Patients and Healthy Controls. Proteomes, 7(2):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Human plasma, vesicles, extracellular vesicles

Jacek R Wisniewski
contact affiliation	Max Planck Institute of Biochemistry, Martinsried, Germany
contact email	jwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliation	Proteomics
contact email	jwisniew@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013234

PRIDE project URI

• PRIDE
  1. PXD013234
     1. Label: PRIDE project
     2. Name: Comparison of proteome composition of serum enriched in extracellular vesicles isolated from polycythemia vera patients and healthy controls