PXD013233

PXD013233 is an original dataset announced via ProteomeXchange.

Title	NFYB-1 regulates mitochondrial function and longevity via lysosomal prosaposin
Description	Mitochondrial activity is critical for cellular vitality and organismal longevity, yet underlying regulatory mechanisms spanning these different levels of organization remain elusive. From RNAi screens for mitochondrial biogenesis, we discovered NFYB-1, a subunit of the NF-Y transcriptional complex, as an ancestral regulator of mitochondrial function. NFYB-1 loss leads to reduced mitochondrial gene expression and oxygen consumption, mitochondrial fragmentation, disruption of mitochondrial stress pathways, and abolition of organismal longevity triggered by mitochondrial impairment. Multi-omics analysis reveals that NFYB-1 is a potent repressor of the ER stress response, as well as lysosomal prosaposin. Surprisingly, limiting prosaposin expression alters ceramide and cardiolipin pools, restores mitochondrial fusion, gene expression and longevity. Thus, the NFYB-1/prosaposin axis coordinates lysosomal to mitochondrial communication to enhance cellular mitochondrial function and organismal health.
HostingRepository	PRIDE
AnnounceDate	2020-03-11
AnnouncementXML	Submission_2021-01-06_06:54:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-26 05:33:35	ID requested
1	2020-03-11 05:34:22	announced
⏵ 2	2021-01-06 06:54:09	announced	2021-01-06: Updated publication reference for PubMed record(s): 32694663.

Tharyan RG, Annibal A, Schiffer I, Laboy R, Atanassov I, Weber AL, Gerisch B, Antebi A, NFYB-1 regulates mitochondrial function and longevity via lysosomal prosaposin. Nat Metab, 2(5):387-396(2020) [pubmed]

curator keyword: Biological

submitter keyword: Mitochondria, ageing, stress response, NFYB-1, prosaposin, cardiolipin, ceramide, transcriptomics, proteomics, metabolomics, label-free, single shot

Adam Antebi
contact affiliation	Max Planck Institute for Biology of Ageing, Cologne, Germany CECAD, University of Cologne, Cologne, Germany
contact email	aantebi@age.mpg.de
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD013233

PRIDE project URI

• PRIDE
  1. PXD013233
     1. Label: PRIDE project
     2. Name: NFYB-1 regulates mitochondrial function and longevity via lysosomal prosaposin