PXD013232

PXD013232 is an original dataset announced via ProteomeXchange.

Title	Cellular concentrations of Protein Kinase D1 in HEK293T and INS-1 cells
Description	Protein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo concentration dependent dimerization in vitro. To test whether the cellular concentration of PKD1 is in the range of the dissociation constant obtained, the expression level of PKD1 was investigated by PRM. To this end, an equimolar mixture of pure, recombinant protein comprising the PKD N-terminus (residues 48-198) and C-terminus (residues 395-892) was used to spike the cell lysates of human HEK293T cells or the rat cell line INS-1. In the latter case, only peptides that are identical between the human and rat PKD1 protein were used to estimate the cellular expression level of PKD1.
HostingRepository	PRIDE
AnnounceDate	2019-08-19
AnnouncementXML	Submission_2019-08-19_00:11:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Hartl
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-26 00:58:51	ID requested
⏵ 1	2019-08-19 00:11:34	announced

Elsner DJ, Siess KM, Gossenreiter T, Hartl M, Leonard TA, A ubiquitin-like domain controls protein kinase D dimerization and activation by trans-autophosphorylation. J Biol Chem, 294(39):14422-14441(2019) [pubmed]

curator keyword: Biological

submitter keyword: protein kinase D, PKD1, HEK293T, INS-1

Thomas A. Leonard
contact affiliation	Department of Structural and Computational Biology, Max F. Perutz Laboratories (MFPL), Campus Vienna Biocenter 5, 1030 Vienna, Austria
contact email	thomas.leonard@meduniwien.ac.at
lab head
Markus Hartl
contact affiliation	Mass Spectrometry Facility, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 3, 1030 Vienna
contact email	markus.hartl@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013232

PRIDE project URI

• PRIDE
  1. PXD013232
     1. Label: PRIDE project
     2. Name: Cellular concentrations of Protein Kinase D1 in HEK293T and INS-1 cells