PXD013224

PXD013224 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome profiling of Coxiella burnetii reveals major metabolic and stress differences under axenic and cell culture cultivation – proteome dataset
Description	Coxiella burnetii, a category B select agent, is endemic worldwide, except New Zealand. It causes annually several outbreaks of the zoonotic disease Q fever predominantly in small ruminants. To date, the lipopolysaccharide (LPS), besides a type IV secretion system (T4SS), is the only defined and characterized virulence determinant of C. burnetii. This surface molecule is used to distinguish between virulent (Ph I) and low-virulent (Ph II) organisms, the latter emerge only after frequent passaging in the laboratory. As an obligate intracellular pathogen, targeted genetic modification is still not a routine and labour intensive procedure. The deeper study of novel determinants is complicated and demands not only advanced techniques for axenic and for cell culture-based cultivation but also novel approaches in the high-resolution mass spectrometry. This work is the first proteomic study comparing. C. burnetii Ph I and Ph II propagated in different axenic media and in cell culture.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:03:11.352.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiri DRESLER
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Coxiella burnetii; NCBI TaxID: NCBITaxon:777; scientific name: Coxiella burnetii RSA 493; NCBI TaxID: 227377;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-25 07:20:39	ID requested
1	2019-10-24 08:23:19	announced
⏵ 2	2024-10-22 04:03:11	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: lipopolysaccharide, type IV secretion system, Q-Exactive, dot/icm, stress response, axenic culture, metabolic activity, quantitative proteomics,Coxiella burnetii

Jiri DRESLER
contact affiliation	Military Health Institute, Prague, Czech Republic
contact email	jiri.dresler@gmail.com
lab head
Jiri DRESLER
contact affiliation	Military Health Institute, Prague/Czech Republic
contact email	jiri.dresler@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD013224

PRIDE project URI

• PRIDE
  1. PXD013224
     1. Label: PRIDE project
     2. Name: Quantitative proteome profiling of Coxiella burnetii reveals major metabolic and stress differences under axenic and cell culture cultivation – proteome dataset