PXD013224 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteome profiling of Coxiella burnetii reveals major metabolic and stress differences under axenic and cell culture cultivation – proteome dataset |
Description | Coxiella burnetii, a category B select agent, is endemic worldwide, except New Zealand. It causes annually several outbreaks of the zoonotic disease Q fever predominantly in small ruminants. To date, the lipopolysaccharide (LPS), besides a type IV secretion system (T4SS), is the only defined and characterized virulence determinant of C. burnetii. This surface molecule is used to distinguish between virulent (Ph I) and low-virulent (Ph II) organisms, the latter emerge only after frequent passaging in the laboratory. As an obligate intracellular pathogen, targeted genetic modification is still not a routine and labour intensive procedure. The deeper study of novel determinants is complicated and demands not only advanced techniques for axenic and for cell culture-based cultivation but also novel approaches in the high-resolution mass spectrometry. This work is the first proteomic study comparing. C. burnetii Ph I and Ph II propagated in different axenic media and in cell culture. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:03:11.352.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jiri DRESLER |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Coxiella burnetii; NCBI TaxID: NCBITaxon:777; scientific name: Coxiella burnetii RSA 493; NCBI TaxID: 227377; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-25 07:20:39 | ID requested | |
1 | 2019-10-24 08:23:19 | announced | |
⏵ 2 | 2024-10-22 04:03:11 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: lipopolysaccharide, type IV secretion system, Q-Exactive, dot/icm, stress response, axenic culture, metabolic activity, quantitative proteomics,Coxiella burnetii |
Contact List
Jiri DRESLER |
contact affiliation | Military Health Institute, Prague, Czech Republic |
contact email | jiri.dresler@gmail.com |
lab head | |
Jiri DRESLER |
contact affiliation | Military Health Institute, Prague/Czech Republic |
contact email | jiri.dresler@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013224
- Label: PRIDE project
- Name: Quantitative proteome profiling of Coxiella burnetii reveals major metabolic and stress differences under axenic and cell culture cultivation – proteome dataset