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PXD013224

PXD013224 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteome profiling of Coxiella burnetii reveals major metabolic and stress differences under axenic and cell culture cultivation – proteome dataset
DescriptionCoxiella burnetii, a category B select agent, is endemic worldwide, except New Zealand. It causes annually several outbreaks of the zoonotic disease Q fever predominantly in small ruminants. To date, the lipopolysaccharide (LPS), besides a type IV secretion system (T4SS), is the only defined and characterized virulence determinant of C. burnetii. This surface molecule is used to distinguish between virulent (Ph I) and low-virulent (Ph II) organisms, the latter emerge only after frequent passaging in the laboratory. As an obligate intracellular pathogen, targeted genetic modification is still not a routine and labour intensive procedure. The deeper study of novel determinants is complicated and demands not only advanced techniques for axenic and for cell culture-based cultivation but also novel approaches in the high-resolution mass spectrometry. This work is the first proteomic study comparing. C. burnetii Ph I and Ph II propagated in different axenic media and in cell culture.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:03:11.352.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJiri DRESLER
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Coxiella burnetii; NCBI TaxID: NCBITaxon:777; scientific name: Coxiella burnetii RSA 493; NCBI TaxID: 227377;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-25 07:20:39ID requested
12019-10-24 08:23:19announced
22024-10-22 04:03:11announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: lipopolysaccharide, type IV secretion system, Q-Exactive, dot/icm, stress response, axenic culture, metabolic activity, quantitative proteomics,Coxiella burnetii
Contact List
Jiri DRESLER
contact affiliationMilitary Health Institute, Prague, Czech Republic
contact emailjiri.dresler@gmail.com
lab head
Jiri DRESLER
contact affiliationMilitary Health Institute, Prague/Czech Republic
contact emailjiri.dresler@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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