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PXD013219 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Flemmingsome reveals an ESCRT-to-membrane coupling required for cytokinesis
DescriptionCytokinesis requires the constriction of ESCRT-III filaments on the midbody side, where abscission occurs. After ESCRT recruitment at the midbody, it is not known how the ESCRT-III machinery localizes to the abscission site. We obtained the proteome of intact, post-abscission midbodies (Flemmingsome) and revealed enriched proteins in this organelle. We propose that the ESCRT-III machinery must be physically coupled to a membrane protein at the cytokinetic abscission site for productive scission, revealing novel common requirements in cytokinesis, exosome formation and retroviral budding.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThibaut Douché
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListL-methionine sulfoxide; ubiquitination signature dipeptidyl lysine; phosphorylated residue; N-acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-25 07:18:12ID requested
12020-05-21 23:54:20announced
Publication List
Addi C, Presle A, Frémont S, Cuvelier F, Rocancourt M, Milin F, Schmutz S, Chamot-Rooke J, Douché T, Duchateau M, Giai Gianetto Q, Salles A, Ménager H, Matondo M, Zimmermann P, Gupta-Rossi N, Echard A, The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis. Nat Commun, 11(1):1941(2020) [pubmed]
Keyword List
submitter keyword: Human, Cytokinesis, Midbody, ESCRT machinery, QExactive, LC-MS/MS
Contact List
Arnaud Echard
contact affiliationMembrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS, 25–28 rue du Dr Roux, F-75015 Paris, France
contact emailarnaud.echard@pasteur.fr
lab head
Thibaut Douché
contact affiliationInstitut Pasteur
contact emailthibaut.douche@pasteur.fr
dataset submitter
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