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PXD013216

PXD013216 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of PKD1 autophosphorylation in vitro and confirmation of stoichiometric S742 phosphorylation of a recombinant PRKD1 kinase domain
DescriptionProtein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo autophosphorylation on serine 742 and potentially also on serine 738 in the activation loop of its kinase domain. We used recombinant PKD1 kinase domain to test whether PKD1 is indeed able to undergo autophosphorylation of its kinase domain and how many sites could be phosphorylated. In order to obtain fully phosphorylated PKD1 kinase domain we adapted our autophosphorylation protocol to obtain monophosphorylated PKD1 kinase domain and determined the phosphorylation site by ArgC digest and phosphopeptide mapping.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:03:06.242.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarkus Hartl
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-25 07:01:15ID requested
12019-08-19 00:09:12announced
22024-10-22 04:03:14announced2024-10-22: Updated project metadata.
Publication List
10.1074/jbc.ra119.008713;
Elsner DJ, Siess KM, Gossenreiter T, Hartl M, Leonard TA, A ubiquitin-like domain controls protein kinase D dimerization and activation by trans-autophosphorylation. J Biol Chem, 294(39):14422-14441(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: PKD1,protein kinase D
Contact List
Thomas A. Leonard
contact affiliationDepartment of Structural and Computational Biology, Max F. Perutz Laboratories (MFPL), Campus Vienna Biocenter 5, 1030 Vienna, Austria
contact emailthomas.leonard@meduniwien.ac.at
lab head
Markus Hartl
contact affiliationMass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 7, 1030 Vienna
contact emailmarkus.hartl@univie.ac.at
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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