PXD013216

PXD013216 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Analysis of PKD1 autophosphorylation in vitro and confirmation of stoichiometric S742 phosphorylation of a recombinant PRKD1 kinase domain
Description	Protein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo autophosphorylation on serine 742 and potentially also on serine 738 in the activation loop of its kinase domain. We used recombinant PKD1 kinase domain to test whether PKD1 is indeed able to undergo autophosphorylation of its kinase domain and how many sites could be phosphorylated. In order to obtain fully phosphorylated PKD1 kinase domain we adapted our autophosphorylation protocol to obtain monophosphorylated PKD1 kinase domain and determined the phosphorylation site by ArgC digest and phosphopeptide mapping.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:03:06.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Hartl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-25 07:01:15	ID requested
1	2019-08-19 00:09:12	announced
⏵ 2	2024-10-22 04:03:14	announced	2024-10-22: Updated project metadata.

Publication List

10.1074/jbc.ra119.008713;
Elsner DJ, Siess KM, Gossenreiter T, Hartl M, Leonard TA, A ubiquitin-like domain controls protein kinase D dimerization and activation by trans-autophosphorylation. J Biol Chem, 294(39):14422-14441(2019) [pubmed]

10.1074/jbc.ra119.008713;

Elsner DJ, Siess KM, Gossenreiter T, Hartl M, Leonard TA, A ubiquitin-like domain controls protein kinase D dimerization and activation by trans-autophosphorylation. J Biol Chem, 294(39):14422-14441(2019) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: PKD1,protein kinase D

curator keyword: Biological

submitter keyword: PKD1,protein kinase D

Contact List

Thomas A. Leonard
contact affiliation	Department of Structural and Computational Biology, Max F. Perutz Laboratories (MFPL), Campus Vienna Biocenter 5, 1030 Vienna, Austria
contact email	thomas.leonard@meduniwien.ac.at
lab head
Markus Hartl
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 7, 1030 Vienna
contact email	markus.hartl@univie.ac.at
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013216

PRIDE project URI

Repository Record List

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