PXD013209

PXD013209 is an original dataset announced via ProteomeXchange.

Title	Homeostatic and Interferon-induced gene expression represent different states of promoter-associated transcription factor ISGF3
Description	Host defense by the innate immune system requires the establishment of antimicrobial states allowing cells to cope with microorganisms before the onset of the adaptive immune response. Interferons (IFN) are of vital importance in the establishment of cell-autonomous antimicrobial immunity. Speed is therefore an important attribute of the cellular response to IFN. With much of the antimicrobial response being installed de novo, this pertains foremost to gene expression, the rapid switch between resting-state and active-state transcription of host defense genes. Mechanisms to meet this demand on the relevant molecular machinery include remodeling of chromatin but also changes in transcription factor interaction prior and during the IFN response. Our results show how distinct transcription factor complexes, determine the responsiveness of Interferon stimulated genes to different IRF9-containing complexes. Raw 264.7 macrophages expressing a doxycycline-inducible, myc-tagged versions of each IRF9-BirA*, STAT2-BirA* and STAT1-BirA* fusion genes were used to study complex formation in vivo. Furthermore, we extended the BioID proximity labeling by coupling it to parallel reaction monitoring to determine the degree and quantity of association between IRF9 and STATs in resting and interferon-treated macrophages.
HostingRepository	PRIDE
AnnounceDate	2019-07-03
AnnouncementXML	Submission_2019-07-08_00:19:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Gossenreiter
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-25 01:53:03	ID requested
1	2019-07-03 01:02:14	announced
⏵ 2	2019-07-08 00:19:16	announced	Updated publication reference for PubMed record(s): 31266943.

Platanitis E, Demiroz D, Schneller A, Fischer K, Capelle C, Hartl M, Gossenreiter T, M, ü, ller M, Novatchkova M, Decker T, A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription. Nat Commun, 10(1):2921(2019) [pubmed]

curator keyword: Biological

submitter keyword: Mouse, LC-MS/MS, BioID

Thomas Decker
contact affiliation	Max F. Perutz Laboratories (MFPL), University of Vienna, Vienna Biocenter (VBC), Austria
contact email	thomas.decker@univie.ac.at
lab head
Thomas Gossenreiter
contact affiliation	Mass Spectrometry Facility, Max F. Perutz Laboratories (MFPL), Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
contact email	thomas.gossenreiter@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013209

PRIDE project URI

• PRIDE
  1. PXD013209
     1. Label: PRIDE project
     2. Name: Homeostatic and Interferon-induced gene expression represent different states of promoter-associated transcription factor ISGF3