PXD013206

PXD013206 is an original dataset announced via ProteomeXchange.

Title	Rice Leaf Proteome with Three 4-plex iTRAQ Experiments by Liquid Chromatograph Mass Spectrometry
Description	Based on ecological crop nutrient deficiency and compensation effect theory, the nitrogen (N)-sensitive stage of rice was selected to study N deficiency at the tillering stage and N compensation at the young panicle differentiation stage. Quantitative proteomics was used to analyze leaf differentially expressed proteins (DEPs), and to investigate the leaf photosynthetic characteristics and yield under N deficiency and N compensation. The results showed that the yield per plant presented an equivalent compensatory effect (CI=1.07). The net photosynthetic rate (Pn), optimal/maximal quantum yield of PSII (Fv/Fm), soil and plant analyzer development (SPAD) value, and glutamic pyruvic transaminase (GPT) activity of T1 (N deficiency at the tillering stage, and N compensation at the young panicle differentiation stage) were lower than those of CK (N at different stages of growth by constant distribution) under N deficiency. However, after N compensation, the Pn, Fv/Fm, SPAD value, and GPT activity increased somewhat. Bioinformatics analysis indicated that DEPs that were enriched in photosynthesis, photosynthesis-antenna proteins, carbon metabolism, and carbon fixation in the photosynthetic organisms pathways were important for the maintenance of cellular homeostasis and metabolic balance in rice when subjected to N deficiency and N compensation. Moreover, the photosynthesis-responsive protein chlorophyll a-b binding protein, ribulose bisphosphate carboxylase small chain, and phosphoglycerate kinase were significantly downregulated under N deficiency. After N compensation, chlorophyll a-b binding protein, NADH dehydrogenase subunit 5, NADH dehydrogenase subunit 7, and peroxidase proteins were significantly upregulated in rice leaves. This study provides a new ecological perspective for N utilization in rice.
HostingRepository	iProX
AnnounceDate	2019-03-24
AnnouncementXML	Submission_2023-03-01_19:44:13.358.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qiangqiang Xiong
SpeciesList	scientific name: Oryza sativa; NCBI TaxID: 4530;
ModificationList	iTRAQ4plex reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-24 19:43:51	ID requested
1	2019-03-24 19:45:35	announced
⏵ 2	2023-03-01 19:44:16	announced	2023-03-02: Update publication information.

Xiong Q, Zhong L, Shen T, Cao C, He H, Chen X, iTRAQ-based quantitative proteomic and physiological analysis of the response to N deficiency and the compensation effect in rice. BMC Genomics, 20(1):681(2019) [pubmed]

submitter keyword: Proteome profiling, N deficiency and the compensation effect, leaf

Xiaorong Chen
contact affiliation	College of Agronomy, Jiangxi Agricultural University
contact email	ccxxrr80@163.com
lab head
Qiangqiang Xiong
contact affiliation	College of Agronomy, Jiangxi Agricultural University
contact email	xqq_qiang@163.com
dataset submitter

iProX dataset URI