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PXD013191

PXD013191 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLGP2 binds PACT to regulate RIG-I- and MDA5-mediated antiviral response
DescriptionThe RIG-I-like receptors (RLRs: RIG-I, MDA5 and LGP2) trigger inflammatory and antiviral responses by sensing non-self RNA molecules produced during viral replication. LGP2 regulation of RIG-I and MDA5-dependant type-I interferon signaling is a matter of controversy. Here we show that LGP2 interacts with different components of the RNA silencing machinery. Particularly, we identified a direct protein-protein interaction between LGP2 and interferon-inducible double-stranded RNA-dependent protein kinase activator A (PACT). The LGP2-PACT interaction is mediated by the regulatory C-terminal domain of LGP2 and is necessary for inhibiting the RIG-I- and amplifying the MDA5-responses. We describe a point mutation within LGP2 that disrupts LGP2-PACT interaction and leads to the loss of LGP2 regulatory activity over RIG-I and MDA5. These results provide a model in which PACT-LGP2 interaction regulates RIG-I and MDA5 inflammatory response and allows cellular RNA silencing machinery to coordinate the innate immune response.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:06:10.305.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThibaut LEGER
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mengo virus; NCBI TaxID: 12107;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-22 04:09:37ID requested
12019-10-02 02:09:52announced
22019-10-07 02:39:13announced2019-10-07: Updated publication reference for PubMed record(s): 31575732.
32024-10-22 04:06:11announced2024-10-22: Updated project metadata.
Publication List
10.1126/scisignal.aar3993;
Sanchez David RY, Combredet C, Najburg V, Millot GA, Beauclair G, Schwikowski B, L, é, ger T, Camadro JM, Jacob Y, Bellalou J, Jouvenet N, Tangy F, Komarova AV, LGP2 binds to PACT to regulate RIG-I- and MDA5-mediated antiviral responses. Sci Signal, 12(601):(2019) [pubmed]
Keyword List
submitter keyword: RIG-1, virus,RNA silencing machinery, PACT, LGP2
Contact List
Dr. Anastassia Komarova
contact affiliationUnité de Génomique Virale et Vaccination, Institut Pasteur, CNRS UMR-3569, Paris, France.
contact emailanastasia.komarova@pasteur.fr
lab head
Thibaut LEGER
contact affiliationEHESP/LERES
contact emailthibaut.leger@ehesp.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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