PXD013191

PXD013191 is an original dataset announced via ProteomeXchange.

Title	LGP2 binds PACT to regulate RIG-I- and MDA5-mediated antiviral response
Description	The RIG-I-like receptors (RLRs: RIG-I, MDA5 and LGP2) trigger inflammatory and antiviral responses by sensing non-self RNA molecules produced during viral replication. LGP2 regulation of RIG-I and MDA5-dependant type-I interferon signaling is a matter of controversy. Here we show that LGP2 interacts with different components of the RNA silencing machinery. Particularly, we identified a direct protein-protein interaction between LGP2 and interferon-inducible double-stranded RNA-dependent protein kinase activator A (PACT). The LGP2-PACT interaction is mediated by the regulatory C-terminal domain of LGP2 and is necessary for inhibiting the RIG-I- and amplifying the MDA5-responses. We describe a point mutation within LGP2 that disrupts LGP2-PACT interaction and leads to the loss of LGP2 regulatory activity over RIG-I and MDA5. These results provide a model in which PACT-LGP2 interaction regulates RIG-I and MDA5 inflammatory response and allows cellular RNA silencing machinery to coordinate the innate immune response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:10.305.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mengo virus; NCBI TaxID: 12107;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-22 04:09:37	ID requested
1	2019-10-02 02:09:52	announced
2	2019-10-07 02:39:13	announced	2019-10-07: Updated publication reference for PubMed record(s): 31575732.
⏵ 3	2024-10-22 04:06:11	announced	2024-10-22: Updated project metadata.

10.1126/scisignal.aar3993;

Sanchez David RY, Combredet C, Najburg V, Millot GA, Beauclair G, Schwikowski B, L, é, ger T, Camadro JM, Jacob Y, Bellalou J, Jouvenet N, Tangy F, Komarova AV, LGP2 binds to PACT to regulate RIG-I- and MDA5-mediated antiviral responses. Sci Signal, 12(601):(2019) [pubmed]

submitter keyword: RIG-1, virus,RNA silencing machinery, PACT, LGP2

Dr. Anastassia Komarova
contact affiliation	Unité de Génomique Virale et Vaccination, Institut Pasteur, CNRS UMR-3569, Paris, France.
contact email	anastasia.komarova@pasteur.fr
lab head
Thibaut LEGER
contact affiliation	EHESP/LERES
contact email	thibaut.leger@ehesp.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD013191

PRIDE project URI

• PRIDE
  1. PXD013191
     1. Label: PRIDE project
     2. Name: LGP2 binds PACT to regulate RIG-I- and MDA5-mediated antiviral response