PXD013186

PXD013186 is an original dataset announced via ProteomeXchange.

Title	The lactococcal phage p2 protein ORF47 impedes Lactococcus lactis MG1363 resistance to phage infection
Description	The lactococcal phage p2 is a model for studying the Skunavirus genus, the most prevalent group of phages in cheese factories worldwide. It infects L. lactis MG1363, a model strain for the study of Gram-positive bacteria. The structural proteins of phage p2 have been thoroughly described. However, most of its non-structural proteins are still uncharacterized. Here, we developed an integrative approach, making use of structural biology, genomics, physiology, and proteomics to provide insights into the function of ORF47, the most conserved non-structural protein of unknown function among the Skunavirus genus. We found this small phage protein to have a major impact on the bacterial proteome and to be important to prevent bacterial resistance to phage infection.
HostingRepository	PRIDE
AnnounceDate	2020-07-30
AnnouncementXML	Submission_2020-07-30_05:46:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Lactococcus lactis subsp. cremoris MG1363; NCBI TaxID: 416870; scientific name: Lactococcus phage; NCBI TaxID: 12374;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-22 03:42:34	ID requested
⏵ 1	2020-07-30 05:46:07	announced

Lemay ML, Maa, ß S, Otto A, Hamel J, Plante PL, Rousseau GM, Tremblay DM, Shi R, Corbeil J, Gagn, é SM, Becher D, Moineau S, A Lactococcal Phage Protein Promotes Viral Propagation and Alters the Host Proteomic Response During Infection. Viruses, 12(8):(2020) [pubmed]

submitter keyword: Phage infection, Lactococcus lactis, mass spectrometry-based proteomics, label-free quantification

Doete Becher
contact affiliation	University of Greifswald Center of Functional Genomics of Microbes Institute of Microbiology Department of Microbial Proteomics Felix-Hausdorff-Str.8 17489 Greifswald Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD013186

PRIDE project URI

• PRIDE
  1. PXD013186
     1. Label: PRIDE project
     2. Name: The lactococcal phage p2 protein ORF47 impedes Lactococcus lactis MG1363 resistance to phage infection