PXD013183

PXD013183 is an original dataset announced via ProteomeXchange.

Title	Proximity-dependent biotinylation by TurboID to identify protein-protein interaction networks in yeast
Description	The use of proximity-dependent biotinylation assays coupled to mass spectrometry (PDB-MS) has changed the field of protein-protein interactions (PPI) studies. Yet, despite the recurrent and successful use of BioID-based PPI screening in mammalian cells, the implementation of PDB-MS in yeast has not been effective. Here we report a simple and rapid approach in yeast to effectively screen for proximal and interacting proteins in their natural cellular environment by using TurboID, a recently described version of the BirA biotin ligase. Using the protein arginine methyltransferase Rmt3 and the RNA exosome subunits, Rrp6 and Dis3, the application of PDB-MS in yeast by using TurboID was able to recover protein-protein interactions previously identified using other biochemical approaches and provided complementary information for a given protein bait. The development of a rapid and effective PDB assay that can systematically analyze PPIs in living yeast cells opens the way for large-scale proteomics studies in this powerful model organism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:16.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danny Bergeron
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-21 10:00:30	ID requested
1	2019-05-15 23:44:59	announced
⏵ 2	2024-10-22 04:06:20	announced	2024-10-22: Updated project metadata.

Larochelle M, Bergeron D, Arcand B, Bachand F, Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast. J Cell Sci, 132(11):(2019) [pubmed]

10.1242/jcs.232249;

submitter keyword: Proximity-dependent biotinylation

TurboID

yeast

S. pombe

Rmt3, RNA exosome

Francois Bachand
contact affiliation	RNA Group, Department of Biochemistry, Universit� de Sherbrooke, Sherbrooke, Qc, Canada
contact email	francois.bachand2@usherbrooke.ca
lab head
Danny Bergeron
contact affiliation	University of Sherbrooke
contact email	danny.bergeron@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013183

PRIDE project URI

• PRIDE
  1. PXD013183
     1. Label: PRIDE project
     2. Name: Proximity-dependent biotinylation by TurboID to identify protein-protein interaction networks in yeast