PXD013176

PXD013176 is an original dataset announced via ProteomeXchange.

Title	Tracking genome-editing and associated molecular perturbations by SWATH mass spectrometry
Description	Advances in gene editing now allow reverse genetics to be applied to a broad range of biological systems. Ultimately, any modification to coding sequences requires confirmation at the protein level, although immunoblotting is often hampered by antibody quality or availability especially in non-mammalian species. Here, we show that Sequential Window Acquisition of All Theoretical Spectra (SWATH) - Mass Spectrometry (MS) is a robust antibody-independent alternative for monitoring gene editing at the protein level and concomitantly defines proteome responses which may provide pertinent biological insights.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:54.002.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qifeng Lin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bicyclus anynana; NCBI TaxID: 110368; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-21 04:46:00	ID requested
1	2019-10-24 02:17:05	announced
2	2019-10-28 01:29:51	announced	2019-10-28: Updated publication reference for PubMed record(s): 31645615.
⏵ 3	2024-10-22 04:07:00	announced	2024-10-22: Updated project metadata.

10.1038/s41598-019-51612-z;

Lin Q, Low LWL, Lau A, Chua EWL, Matsuoka Y, Lian Y, Monteiro A, Tate S, Gunaratne J, Carney TJ, Tracking genome-editing and associated molecular perturbations by SWATH mass spectrometry. Sci Rep, 9(1):15240(2019) [pubmed]

submitter keyword: CRISPR,SWATH, gene editing

Jayantha Gunaratne
contact affiliation	Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)
contact email	jayanthag@imcb.a-star.edu.sg
lab head
Qifeng Lin
contact affiliation	Institute of Molecular & Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR)
contact email	qflin@imcb.a-star.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD013176

PRIDE project URI

• PRIDE
  1. PXD013176
     1. Label: PRIDE project
     2. Name: Tracking genome-editing and associated molecular perturbations by SWATH mass spectrometry