PXD013173 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio) |
Description | Lysine succinylation (Ksu) is a novel identified post-translational modification that conserved from prokaryote to eukaryotes. As a kind of acylation, Ksu was reported to have different functions with others acylation at lysine residue. However, recently studies on the Ksu mainly focus on the plants and bacterial, there are still very rare studies in the vertebrate. Therefore, the biological role of succinylation remains largely unknown in mammal. In this study, we performed global Ksu mapping in Danio rerio (zebrafish) using mass spectrometry-based proteomics with enrichment of Ksu peptides by immunoprecipitation technology. As a result, we totally identified 552 Ksu sites in 164 proteins. Compared with our previous studies on lysine acetylation and crotonylation, Ksu plays a major role in a diverse metabolic process, such as carbon metabolism and tricarboxylic acid circle. In addition, we defined 5 new succinylation motifs: (su)KA, (suc)KxxxxA, (su)KxxxxL, (su)KxA, (su)KxV. In conclusion, our result provides proteome-wide database for study of Ksu in zebrafish and our bioinformatics result facilitated the understanding of the Ksu in the role of central metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-27 |
AnnouncementXML | Submission_2019-11-27_05:59:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yan Gao |
SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | succinylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-21 02:22:38 | ID requested | |
1 | 2019-09-20 08:26:29 | announced | |
⏵ 2 | 2019-11-27 05:59:40 | announced | 2019-11-27: Updated project metadata. |
Publication List
Gao Y, Lee H, Kwon OK, Tan M, Kim KT, Lee S, ). J Proteome Res, 18(10):3762-3769(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Zebrafish larvae,LC-MS/MS, lysine succinylation |
Contact List
Sangkyu Lee |
contact affiliation | Associate Professor College of Pharmacy, Kyungpook National University |
contact email | pharm239@gmail.com |
lab head | |
Yan Gao |
contact affiliation | Kyungpook National University |
contact email | gaoyan0723@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013173
- Label: PRIDE project
- Name: Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio)