PXD013172

PXD013172 is an original dataset announced via ProteomeXchange.

Title	Differential proteomics of the synaptosomal fraction from rat dentate gyrus identified the changes associated with aging-dependent cognitive decline
Description	The study contained three groups of Sprague-Dawley male rats – two groups of aging animals (22-24 months) separated based on performance in spatial reference memory task; and the third, control group of young animals () not separated by behavioural testing. The synaptosome – enriched membrane fraction of the dentate gyrus was isolated from nine randomly chosen individuals per group and analysed by tandem mass tag (TMT) labelling and mass spectrometry (MS)-based quantitative proteomics. The proteomic experiment was performed in three TMT10plex sets, with each set containing samples from three biological replicates per group and one TMT 126 reference channel reserved to directly compare the protein intensities across the entire study. The peptides were analysed in two parallel MS runs representing two technical replicates per biological sample. The total number of 6513 protein entries was identified of which 471 showed a significantly changed level (p<0.05) in any of the three animal groups. The comparison between aged and young animals revealed 293 significantly changed proteins (p<0.05) in aged good performers vs young rats, and 310 significant proteins (p < 0.05) in aged bad performers vs young ones. The proteomic differences between aged good vs bad performers were represented by 78 significantly changed proteins (p < 0.05).
HostingRepository	PRIDE
AnnounceDate	2019-08-19
AnnouncementXML	Submission_2019-08-19_01:07:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gert Lubec
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-21 02:17:32	ID requested
⏵ 1	2019-08-19 01:07:51	announced

Lubec J, Smidak R, Malikovic J, Feyissa DD, Korz V, H, ö, ger H, Lubec G, Dentate Gyrus Peroxiredoxin 6 Levels Discriminate Aged Unimpaired From Impaired Rats in a Spatial Memory Task. Front Aging Neurosci, 11():198(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: dentate gyrus, spatial reference memory, quantitative proteomics

Gert Lubec
contact affiliation	Paracelsus Private Medical University, Salzburg, Austria
contact email	gert.lubec@lubeclab.com
lab head
Gert Lubec
contact affiliation	Paracelsus Private Medical University
contact email	gert.lubec@lubeclab.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013172

PRIDE project URI

• PRIDE
  1. PXD013172
     1. Label: PRIDE project
     2. Name: Differential proteomics of the synaptosomal fraction from rat dentate gyrus identified the changes associated with aging-dependent cognitive decline