PXD013168

PXD013168 is an original dataset announced via ProteomeXchange.

Title	Escherichia coli can adapt its protein translocation machinery for enhanced periplasmic recombinant protein production
Description	Recently, we engineered a tunable rhamnose promoter-based setup for the production of recombinant proteins in E. coli. This setup enabled us to show that being able to precisely set the production rate of a secretory recombinant protein is critical to enhance protein production yields in the periplasm. It is assumed that precisely setting the production rate of a secretory recombinant protein is required to harmonize its production rate with the protein translocation capacity of the cell. Here, using proteome analysis we show that enhancing periplasmic production of human Growth Hormone (hGH) using the tunable rhamnose promoter-based setup is accompanied by increased accumulation levels of at least three key players in protein translocation; the peripheral motor of the Sec-translocon SecA, leader peptidase (LepB) and the cytoplasmic membrane protein integrase/chaperone YidC. Thus, enhancing periplasmic hGH production leads to increased Sec-translocon capacity, increased capacity to cleave signal peptides from secretory proteins and an increased capacity of an alternative membrane protein biogenesis pathway, which frees up Sec-translocon capacity for protein secretion. When cells with enhanced periplasmic hGH production yields were harvested and subsequently cultured in the absence of inducer, SecA, LepB and YidC levels went down again. This indicates that when using the tunable rhamnose-promoter system to enhance the production of a protein in the periplasm, E. coli can adapt its protein translocation machinery for enhanced recombinant protein production in the periplasm.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:52.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarzyna Dolata
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-20 09:25:41	ID requested
1	2020-03-05 08:41:33	announced
⏵ 2	2024-10-22 04:07:00	announced	2024-10-22: Updated project metadata.

10.3389/fbioe.2019.00465;

Karyolaimos A, Dolata KM, Antelo-Varela M, Mestre Borras A, Elfageih R, Sievers S, Becher D, Riedel K, de Gier JW, Can Adapt Its Protein Translocation Machinery for Enhanced Periplasmic Recombinant Protein Production. Front Bioeng Biotechnol, 7():465(2019) [pubmed]

curator keyword: Technical, Biological

submitter keyword: E.coli, Recombinant protein production, protein secretion

Katarzyna Dolata
contact affiliation	Katarzyna Dolata, University of Greifswald Katarzyna Dolata, Katharina Riedel, Institute of Microbiology, Departmentof Microbial Physiology and Molecular Biology Ernst-Moritz-Arndt-University Greifswald Felix-Hausdorff-Straße 8 17489 Greifswald ( lab head )
contact email	dolatak@uni-greifswald.de
lab head
Katarzyna Dolata
contact affiliation	University of Greifswald
contact email	dolatak@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD013168

PRIDE project URI

• PRIDE
  1. PXD013168
     1. Label: PRIDE project
     2. Name: Escherichia coli can adapt its protein translocation machinery for enhanced periplasmic recombinant protein production