PXD013167

PXD013167 is an original dataset announced via ProteomeXchange.

Title	The hematopoietic master transcription factor PU.1 requires its interaction with the SWI/SNF remodeler to access chromatin de novo
Description	PU.1 is a prototype master transcription factor (TF) of hematopoietic cell differentiation with diverse roles in different lineages. Analysis of its genome-wide binding pattern across PU.1 expressing cell types revealed manifold cell type-specific binding patterns. They are not consistent with the epigenetic and chromatin constraints to PU.1 binding observed in vitro, suggesting that PU.1 requires auxiliary factors to access DNA in vivo. Using a model of transient mRNA expression we show that PU.1 induction leads to the extensive remodeling of chromatin, redistribution of partner transcription factors and rapid initiation of a myeloid gene expression program in heterologous cell types. By probing PU.1 mutants for defects in chromatin access and screening for PU.1 proximal proteins in vivo, we found that its N-terminal acidic domain was required for the recruitment of SWI/SNF remodeling complexes, de novo chromatin access and stable binding as well as the redistribution of partner TFs.
HostingRepository	PRIDE
AnnounceDate	2019-11-20
AnnouncementXML	Submission_2020-01-29_05:47:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-20 09:19:43	ID requested
1	2019-11-20 07:31:05	announced
⏵ 2	2020-01-29 05:47:31	announced	2020-01-29: Updated publication reference for PubMed record(s): 31964861.

Minderjahn J, Schmidt A, Fuchs A, Schill R, Raithel J, Babina M, Schmidl C, Gebhard C, Schmidhofer S, Mendes K, Ratermann A, Glatz D, N, ü, tzel M, Edinger M, Hoffmann P, Spang R, L, ä, ngst G, Imhof A, Rehli M, Mechanisms governing the pioneering and redistribution capabilities of the non-classical pioneer PU.1. Nat Commun, 11(1):402(2020) [pubmed]

submitter keyword: transcription factor, cell differenciation, chromatin remodelling

Axel Imhof
contact affiliation	Biomedical Center, LMU Munich Molecular Biology Großhaderner Strasse 9 82152 Planegg-Martinsried Phone: (089) 2180 75420
contact email	imhof@lmu.de
lab head
Andreas Schmidt
contact affiliation	Biomedical Center, LMU
contact email	A.Schmidt@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013167

PRIDE project URI

• PRIDE
  1. PXD013167
     1. Label: PRIDE project
     2. Name: The hematopoietic master transcription factor PU.1 requires its interaction with the SWI/SNF remodeler to access chromatin de novo