PXD013146

PXD013146 is an original dataset announced via ProteomeXchange.

Title	Multi-OMIC analysis of brain and serum from chronically-stressed mice
Description	Major depressive disorder (MDD) is a complex condition with unclear pathophysiology. Molecular disruptions within the periphery and limbic brain regions contribute to depression symptomatology. Here, we utilized a mouse chronic stress model of MDD and performed metabolomic, lipidomic, and proteomic profiling on serum plus several brain regions (ventral hippocampus, nucleus accumbens, and prefrontal cortex) of susceptible, resilient, and unstressed control mice. Proteomic analysis identified three serum proteins reduced in susceptible animals; lipidomic analysis detected differences in lipid species between resilient and susceptible animals in serum and brain; and metabolomic analysis revealed pathway dysfunctions of purine metabolism, beta oxidation, and antioxidants, which were differentially associated with stress susceptibility vs resilience by brain region. Antidepressant treatment ameliorated MDD-like behaviors and affected key metabolites within outlined networks, most dramatically in the ventral hippocampus. This work presents a resource for chronic stressinduced, tissue-specific changes in proteins, lipids, and metabolites and illuminates how molecular dysfunctions contribute to individual differences in stress sensitivity
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_09:18:42.445.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Punit Shah
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-19 07:23:42	ID requested
⏵ 1	2021-09-08 09:18:43	announced

Hamilton PJ, Chen EY, Tolstikov V, Pe, ñ, a CJ, Picone JA, Shah P, Panagopoulos K, Strat AN, Walker DM, Lorsch ZS, Robinson HL, Mervosh NL, Kiraly DD, Sarangarajan R, Narain NR, Kiebish MA, Nestler EJ, Chronic stress and antidepressant treatment alter purine metabolism and beta oxidation within mouse brain and serum. Sci Rep, 10(1):18134(2020) [pubmed]

submitter keyword: Mouse Serum

Punit Shah
contact affiliation	Senior Scientist
contact email	Punit.shah@berghealth.com
lab head
Punit Shah
contact affiliation	Berg
contact email	punit.shah@berghealth.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD013146

PRIDE project URI

• PRIDE
  1. PXD013146
     1. Label: PRIDE project
     2. Name: Multi-OMIC analysis of brain and serum from chronically-stressed mice