PXD013144

PXD013144 is an original dataset announced via ProteomeXchange.

Title	Structural basis for assembly of lamin at the molecular level
Description	Nuclear structure and function are governed by lamins, which are intermediate filaments mostly consisting of alpha-helices. Different lamin assembly models have been proposed based on low resolution or fragmented structures. However, their assembly mechanisms at the molecular level are poorly understood. Here, we present a crystal structure of a long human lamin fragment at 3.2 Å resolution, which visualized the full-length features. The structure presented the anti-parallel arrangement of two coiled-coil dimers, which was important for the assembly process. We further discovered a new interaction between the lamin dimers by using chemical cross-linking and mass analysis. Based on these two interactions we proposed a molecular mechanism of lamin assembly, which agreed well with the recent model representing the native state, and could explain pathological mutations. Our findings provide the structural information to understand molecular mechanisms of assembly of intermediate filaments, and molecular insights into nuclear functions and aging process.
HostingRepository	PRIDE
AnnounceDate	2019-08-26
AnnouncementXML	Submission_2019-08-26_00:51:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong-Hak Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-cysteine methyl ester; L-lysine amide
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-19 06:23:29	ID requested
⏵ 1	2019-08-26 00:51:18	announced

Ahn J, Jo I, Kang SM, Hong S, Kim S, Jeong S, Kim YH, Park BJ, Ha NC, Structural basis for lamin assembly at the molecular level. Nat Commun, 10(1):3757(2019) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biological

submitter keyword: Nuclear lamin protein, filament assembly structure, X-ray crystallography, chemical crosslinking, gel-based LC-MSMS

Yong-Hak Kim
contact affiliation	Daegu Catholic University School of Medicine
contact email	ykim@cu.ac.kr
lab head
Yong-Hak Kim
contact affiliation	Catholic University of Daegu School of Medicine
contact email	ykim@cu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013144
     1. Label: PRIDE project
     2. Name: Structural basis for assembly of lamin at the molecular level