PXD013133
PXD013133 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LFQ_K5D1-cTEC_K5D1-beta5tKO-cTEC |
| Description | Cortical TECs from K5D1 and K5D1-β5tKO mice were lysed, reduced, alkylated, and subjected to methanol/chloroform precipitation. Proteins were digested with trypsin/Lys-C mix. The digests were desalted using GL-Tip SDB, and the eluates were evaporated in a SpeedVac concentrator and dissolved in 0.1% TFA. LC-MS/MS analysis of the resultant peptides (400 ng each) was performed on an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer. The mass spectrometer was operated in a data-dependent acquisition mode with a top 10 MS/MS method. MS1 spectra were measured with a resolution of 70,000, an AGC target of 1×106 and a mass range from 350 to 1,500 m/z. HCD MS/MS spectra were acquired at a resolution of 17,500, an AGC target of 5×104, an isolation window of 2.0 m/z, a maximum injection time of 60 msec and a normalized collision energy of 27. Dynamic exclusion was set to 10 sec. Raw data were directly analyzed against the Swiss-Prot database restricted to Mus musculus using Proteome Discoverer version 2.2 with Mascot search engine version 2.5 for identification and label-free precursor ion quantification. Peptides and proteins were filtered at a FDR of 1% using the percolator node and the protein FDR validator node, respectively. Normalization was performed such that the total sum of abundance values for each sample over all peptides was the same. |
| HostingRepository | jPOST |
| AnnounceDate | 2019-10-08 |
| AnnouncementXML | Submission_2022-09-18_03:49:43.166.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hidetaka Kosako |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-03-18 22:40:50 | ID requested | |
| 1 | 2019-10-07 22:37:54 | announced | |
| 2 | 2019-10-07 22:46:52 | announced | 2019-10-08: Updated announcement date. |
| 3 | 2020-01-09 18:32:42 | announced | 2020-01-10: Updated PubMed. |
| 4 | 2021-02-04 17:01:23 | announced | 2021-02-04: Updated FTP location. |
| ⏵ 5 | 2022-09-18 03:49:43 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Ohigashi I, Tanaka Y, Kondo K, Fujimori S, Kondo H, Palin AC, Hoffmann V, Kozai M, Matsushita Y, Uda S, Motosugi R, Hamazaki J, Kubota H, Murata S, Tanaka K, Katagiri T, Kosako H, Takahama Y, Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells. Cell Rep, 29(9):2901-2916.e6(2019) [pubmed] |
Keyword List
| submitter keyword: LFQ |
Contact List
| Hidetaka Kosako | |
|---|---|
| lab head | |
| Hidetaka Kosako | |
| contact affiliation | Tokushima University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000569/ |
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