PXD013130

PXD013130 is an original dataset announced via ProteomeXchange.

Title	Sensing and signalling of DNA breaks in extracts of preblastoderm Drosophila embryos
Description	Preblastoderm Drosophila embryos replicate their genomes at maximal speed. The assembly of chromatin on the newly synthesized chromosomes relies entirely on high concentrations of maternal nucleosome assembly and remodelling factors. Extracts from preblastoderm embryos can be turned into a system for highly efficient assembly of complex chromatin. We serendipitously discovered that this chromatin assembly system contains numerous activities that recognise unprotected DNA ends and initiate DNA damage signalling cascades. Within minutes the phosphorylation of H2A.V (homologous to H2A.X) initiates from DNA breaks and spreads over tens of thousands DNA base pairs. The phosphorylation of H2A.V remains tightly associated with the damaged DNA in cis and does not spread to intact DNA circles in the same reaction. The ultrafast cycles of syncytial nuclei divisions of cleavage-stage embryos do not allow sufficient time to repair DNA breaks. Rather, fatally damaged nuclei are eliminated by a programmed ‘mitotic catastrophe’. Our descriptions of the damage-related proteome and DNA break-dependent phospho-proteome suggest that certain aspects of the DNA damage checkpoint that leads to the elimination of nuclei can be studied in this cell-free system. Our data provide a rich resource for in-depth mechanistic analyses of the DNA break response in this model system.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:35.197.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Schmidt
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-18 09:09:47	ID requested
1	2019-10-14 16:42:02	announced
⏵ 2	2024-10-22 04:05:36	announced	2024-10-22: Updated project metadata.

10.1093/nar/gkz473;

Harpprecht L, Baldi S, Schauer T, Schmidt A, Bange T, Robles MS, Kremmer E, Imhof A, Becker PB, A Drosophila cell-free system that senses DNA breaks and triggers phosphorylation signalling. Nucleic Acids Res, 47(14):7444-7459(2019) [pubmed]

submitter keyword: DNA repair,DNA damage response, D.melanogaster

Axel Imhof
contact affiliation	Protein Analysis Unit ZfP BioMedical Center, LMU Munich Grosshaderner Strasse 9 82152 Planegg-Martinsried Tel. +49 89 2180 75420
contact email	imhof@lmu.de
lab head
Andreas Schmidt
contact affiliation	CECAD, University of Cologne, AG Krüger
contact email	A.Schmidt@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD013130

PRIDE project URI

• PRIDE
  1. PXD013130
     1. Label: PRIDE project
     2. Name: Sensing and signalling of DNA breaks in extracts of preblastoderm Drosophila embryos