PXD013127

PXD013127 is an original dataset announced via ProteomeXchange.

Title	786-O ubiquitiome
Description	786-O WT and BAP1-KO cells were cultured in heavy (13C6-L-arginine, 13C6-L-lysine, Cambridge Isotope Lab) and light (unlabeled L-arginine and L-lysine) SILAC RPMI-1640 medium (Thermo Fisher Scienific), respectively. After more than 10 passages, cells were lysed with 8 M urea and 5 mg protein from each group were mix. The ubiquitinated peptide enrichment was performed using PTMScan® (Cell Signaling Technology, #5562) following the manufacturer’s instructions.
HostingRepository	PRIDE
AnnounceDate	2019-04-18
AnnouncementXML	Submission_2019-04-18_02:08:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pengsheng Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-18 08:36:25	ID requested
⏵ 1	2019-04-18 02:08:55	announced

Chen P, Wang H, Zhang W, Chen Y, Lv Y, Wu D, Guo M, Deng H, Loss of BAP1 Results in Growth Inhibition and Enhances Mesenchymal-Epithelial Transition in Kidney Tumor Cells. Mol Cell Proteomics, 18(7):1320-1329(2019) [pubmed]

submitter keyword: ccRCC 786-O ubiquitiome

Haiteng Deng
contact affiliation	Shool of Life Sciences, Tsinghua University, China
contact email	dht@mail.tsinghua.edu.cn
lab head
Pengsheng Chen
contact affiliation	Tsinghua Univ
contact email	878357025@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD013127

PRIDE project URI

• PRIDE
  1. PXD013127
     1. Label: PRIDE project
     2. Name: 786-O ubiquitiome