PXD013111

PXD013111 is an original dataset announced via ProteomeXchange.

Title	The Urinary Peptidome Identifies Prognostic Biomarkers for Small Renal Masses
Description	Purpose: Renal cell carcinoma (RCC) is often diagnosed incidentally as an early-stage small renal mass (SRM; pT1a, ≤ 4 cm). Increasing concerns surrounding the overtreatment of patients with benign or clinically indolent tumors has led to a shift in current treatment recommendations, especially for elderly and infirm patients. There are currently no available biomarkers to accurately stratify patients according to risk. Therefore, we set out to identify early biomarkers of RCC progression. Experimental Design: We employed label-free LC-MS/MS and targeted parallel-reaction monitoring (PRM) to identify early, non-invasive diagnostic and prognostic biomarkers for early-stage RCC-SRMs. In total, we evaluated 115 urine samples, including 33 renal oncocytoma (≤ 4 cm) cases, 30 progressive and 26 non-progressive clear cell RCC-SRM (ccRCC-SRM) cases, in addition to 26 healthy controls. Results: We identified nine endogenous peptides which displayed significantly elevated expression in ccRCC-SRMs relative to healthy controls. Peptides NVINGGSHAGNKLAMQEF, VNVDEVGGEALGRL, and VVAGVANALAHKYH showed significantly elevated expression in ccRCC-SRMs relative to renal oncocytoma. Additionally, peptides SHTSDSDVPSGVTEVVVKL and IVDNNILFLGKVNRP displayed significantly elevated expression in progressive relative to non-progressive ccRCC-SRMs. Peptide SHTSDSDVPSGVTEVVVKL showed the most significant discriminatory ability (AUC: 0.76, 95% CI: 0.62 to 0.90, p = 0.0027). Patients with elevated SHTSDSDVPSGVTEVVVKL expression had significantly shorter overall survival (HR: 4.13, 95% CI: 1.09 to 15.65, p = 0.024) compared to patients with lower expression. Conclusions: Our in-depth peptidomic analysis identified novel biomarkers for early-stage RCC-SRMs. Characterization of urinary peptides may provide insight into early RCC progression and could potentially help assign patients to appropriate management programs.
HostingRepository	PRIDE
AnnounceDate	2019-08-08
AnnouncementXML	Submission_2019-08-08_07:57:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ashley Di Meo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-18 02:11:21	ID requested
⏵ 1	2019-08-08 07:57:47	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Peptidomics, mass spectrometry, urine, clear cell renal cell carcinoma, small renal mass

Eleftherios P. Diamandis
contact affiliation	Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Canada
contact email	Eleftherios.diamandis@sinaihealthsystem.ca
lab head
Ashley Di Meo
contact affiliation	University of Toronto
contact email	ashley.dimeo@mail.utoronto.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013111
     1. Label: PRIDE project
     2. Name: The Urinary Peptidome Identifies Prognostic Biomarkers for Small Renal Masses