PXD013102 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Systematic characterization of BAF mutations explains intra-complex synthetic lethalities |
Description | Aberrations in genes coding for subunits of the BAF chromatin remodeling complex are highly abundant in human cancers. Currently, it is not understood how these loss-of-function mutations contribute to cancer development and how they can be targeted therapeutically. The cancer type specific occurrence patterns of certain subunit mutations suggest subunit-specific effects on BAF complex function, possibly by the formation of aberrant residual complexes. Here, we systematically characterize the effects of individual subunit loss on complex composition, chromatin accessibility and gene expression in a panel of knock-out cell lines deficient for 22 targetable BAF subunits. We observe strong, specific and often discordant alterations dependent on the targeted subunit and show that these explain intra-complex co-dependencies, including the novel synthetic lethal interactions SMARCA4-ARID2, SMARCA4-ACTB and SMARCC1-SMARCC2. These data provide insights into the role of different BAF subcomplexes in genome-wide chromatin organization and suggest novel approaches to therapeutically target BAF mutant cancers. |
HostingRepository | PRIDE |
AnnounceDate | 2019-08-01 |
AnnouncementXML | Submission_2019-08-01_04:09:02.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD013102 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Peter Májek |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Phospho; Methyl; TMT6plex; Oxidation; Acetyl; Carbamidomethyl |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-15 04:41:29 | ID requested | |
⏵ 1 | 2019-08-01 04:09:03 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: BAF complex, mammalian SWI/SNF complex, chromatin remodeling, interaction proteomics, ATAC-seq, transcriptomics, synthetic lethality, chromatin accessibility, chromatin binding |
Contact List
Stefan Kubicek |
contact affiliation | CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Christian Doppler Laboratory for Chemical Epigenetics and Anti-Infectives Lazarettgasse 14, AKH BT 25.3 1090 Vienna, Austria |
contact email | SKubicek@cemm.oeaw.ac.at |
lab head | |
Peter Májek |
contact affiliation | CeMM, Reserach Center for Molecular Medicine |
contact email | pmajek@cemm.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013102
- Label: PRIDE project
- Name: Systematic characterization of BAF mutations explains intra-complex synthetic lethalities