PXD013097

PXD013097 is an original dataset announced via ProteomeXchange.

Title	PhAXA-MS analysis of 4EBP1, cJun, and ERK2 Kinases
Description	Recent estimates of the human proteome suggest that there are ~20,000 protein-coding genes, the protein products of which contain >145,000 phosphorylation sites. Unfortunately, in-depth examination of the human phosphoproteome has far outpaced the ability to annotate the kinases that mediate these post-translational modifications. To obtain actionable information about phosphorylation-driven signaling cascades, it is essential to identify the kinases responsible for phosphorylating sites that differ across disease states. To fill in these knowledge gaps, we have developed an unbiased, chemoproteomic approach for identifying high confidence kinase-substrate interactions with phosphosite specificity. Using this assay, we have uncovered the role of cyclin-dependent kinase 4 (CDK4), a clinically validated kinase important for cell cycle progression, in regulating cap-dependent translation via phosphorylation of the tumor suppressor 4E-BP1.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:54.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dylan Mitchell
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-14 06:51:57	ID requested
1	2019-05-04 03:24:27	announced
⏵ 2	2024-10-22 04:10:54	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Chemoproteomics, cJun, 4E-BP1, ERK2, kinases, activity based probes, crosslinking

Amanda Lee Garner
contact affiliation	Department of Medicinal Chemistry, University of Michigan, United States of America
contact email	algarner@umich.edu
lab head
Dylan Mitchell
contact affiliation	University of Michigan
contact email	dylancm@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013097

PRIDE project URI

• PRIDE
  1. PXD013097
     1. Label: PRIDE project
     2. Name: PhAXA-MS analysis of 4EBP1, cJun, and ERK2 Kinases