PXD013093

PXD013093 is an original dataset announced via ProteomeXchange.

Title	Systematic Discovery of Deeply Conserved Protein Complexes, Selaginella
Description	The associated files are mass spec data from two HPLC fractionations (Size Exclusion (SEC) or a triple phase ion exchange combination (WaxWaxCat)) of a single native protein extract from Selaginella moellendorfii. The extract was made from frozen powdered green tissue in 50 mM Tris pH 7.5, 150 mM NaCl, 5 mM EGTA, 10% glycerol, 1% NP40, phosphatase inhibitors and plant-specific protease inhibitors. 1 mg total protein was separated by SEC and 1.25 mg (diluted to 50 mM NaCl) by a concatenated series of three columns: PolyWaxLP, PolyWaxLP, and poly CAT A (PolyLC, Inc). The triple column series was eluted with a NaCl gradient. The ion exchange fractions # 55-60 were not processed for mass spectrometry becaue the A280 trace from the HPLC indicated insufficient protein present in those fractions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:09.339.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013093
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ophelia Papoulas
SpeciesList	scientific name: Selaginella moellendorffii; NCBI TaxID: 88036;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-14 05:33:44	ID requested
1	2019-10-28 06:53:18	announced
⏵ 2	2023-11-14 08:50:09	announced	2023-11-14: Updated project metadata.

10.6019/PXD013093;

curator keyword: Biological

submitter keyword: Spike moss,Selaginella moellendorffii, CF-MS, cofractionation mass spec

Edward M. Marcotte
contact affiliation	Molecular Biosciences and the Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, USA
contact email	marcotte@austin.utexas.edu
lab head
Ophelia Papoulas
contact affiliation	University of Texas at Austin
contact email	papoulas@austin.utexas.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD013093

PRIDE project URI

• PRIDE
  1. PXD013093
     1. Label: PRIDE project
     2. Name: Systematic Discovery of Deeply Conserved Protein Complexes, Selaginella