PXD013087

PXD013087 is an original dataset announced via ProteomeXchange.

Title	Comparative membrane-associated proteome of Leishmania (Leishmania) infantum and L.(L.) amazonensis
Description	Protozoan parasites of the genus Leishmania are causative agents of leishmaniasis, a wide range of diseases affecting 12 million people worldwide. The species L. (L.) infantum and L. (L.) amazonensis are causative agents of visceral and cutaneous leishmaniasis, respectively. Most proteome analyses of Leishmania have been carried out on whole-cell extracts. However, this approach tends to underrepresent membrane-associated proteins because of their high hydrophobicity and low solubility. Due to the great importance of membrane-associated proteins in biological processes, including host–parasite interactions, virulence and invasiveness, this study applied label-free shotgun proteomics to characterize and evaluate abundance levels of plasma membrane sub-proteome of promastigotes life-stage. The total number of proteins identified in L. (L.) infantum and L. (L.) amazonensis was 2033 and 2243, respectively. Both species shared 1908 of these quantified proteins. After cell localization prediction of all identified proteins, 394 proteins were described as plasma membrane-associated proteins and their majority (320 proteins) was presented in both species. Considering only exclusive proteins, 18 proteins were detected only in L. (L.) infantum and 56 proteins in L. (L.) amazonensis. We used two criteria to define “regulated” proteins; i) proteins with p-value < 0.05 after One-Way ANOVA analysis (quantitative analysis) and proteins detected only in L. (L.) infantum or L. (L.) amazonensis (qualitative analysis).
HostingRepository	PRIDE
AnnounceDate	2022-02-11
AnnouncementXML	Submission_2022-02-11_06:42:36.796.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiago Verano-Braga
SpeciesList	scientific name: Leishmania infantum JPCM5; NCBI TaxID: 435258; scientific name: Leishmania mexicana; NCBI TaxID: 5665;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-13 08:48:52	ID requested
⏵ 1	2022-02-11 06:42:37	announced

Dataset with its publication pending

submitter keyword: Leishmania (Leishmania) infantum, Leishmania (Leishmania) amazonensis, label free proteomics, plasma membrane proteins

Thiago Verano-Braga
contact affiliation	Departamento de Fisiologia e Biofisica, Universidade Federal de Minas Gerais
contact email	tvbraga@icb.ufmg.br
lab head
Thiago Verano-Braga
contact affiliation	Federal University of Minas Gerais
contact email	npf.ufmg@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013087
     1. Label: PRIDE project
     2. Name: Comparative membrane-associated proteome of Leishmania (Leishmania) infantum and L.(L.) amazonensis