PXD013086

PXD013086 is an original dataset announced via ProteomeXchange.

Title	Docosahexaenoic acid attenuates metabolic dysfunctions induced by lipopolysaccharide in BV-2 microglial cells
Description	Microglial cell activation has been linked to many neurodegenerative diseases. Upon stimulation by lipopolysaccharide (LPS), a number of proteins involved in inflammatory and oxidative pathways are activated. Production of nitric oxide has been regarded as a signature marker of inflammatory responses. Our recent studies demonstrated the effects of docosahexaenoic acid (DHA) to inhibit the LPS-induced inflammatory responses in BV-2 microglial cells. DHA also can upregulate the anti-oxidative pathway involving nuclear factor erythroid 2-Like 2 (Nrf2) and synthesis of heme oxygenase-1 (HO-1), a potent anti-oxidative enzyme. In order to further understand the proteins involved, this study used a label-free quantitative proteomics approach to examine effects of DHA and LPS on proteins and signaling pathways in microglial cells.
HostingRepository	PRIDE
AnnounceDate	2020-04-20
AnnouncementXML	Submission_2020-07-14_23:29:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013086
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bo Yang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-13 08:31:54	ID requested
1	2020-04-19 23:49:21	announced
⏵ 2	2020-07-14 23:29:01	announced	2020-07-15: Updated publication reference for PubMed record(s): 32302149.

Yang B, Li R, Liu PN, Geng X, Mooney BP, Chen C, Cheng J, Fritsche KL, Beversdorf DQ, Lee JC, Sun GY, Greenlief CM, Quantitative Proteomics Reveals Docosahexaenoic Acid-Mediated Neuroprotective Effects in Lipopolysaccharide-Stimulated Microglial Cells. J Proteome Res, 19(6):2236-2246(2020) [pubmed]

submitter keyword: docosahexaenoic acid, microglia, lipopolysaccharide, label-free quantification, ion mobility, TIMS, time-of-flight.

C. Michael Greenlief
contact affiliation	Department of Chemistry, University of Missouri-Columbia
contact email	greenliefm@missouri.edu
lab head
Bo Yang
contact affiliation	University of Missouri-Columbia
contact email	bykm5@mail.missouri.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013086
     1. Label: PRIDE project
     2. Name: Docosahexaenoic acid attenuates metabolic dysfunctions induced by lipopolysaccharide in BV-2 microglial cells