PXD013081

PXD013081 is an original dataset announced via ProteomeXchange.

Title	The type VII secretion system protects Staphylococcus aureus against host-specific fatty acids
Description	The Staphylococcus aureus type VII secretion system (T7SS) exports several proteins that are pivotal for bacterial virulence. The mechanisms underlying T7SS-mediated staphylococcal survival during infection are nevertheless unclear. Here we show that EsxC, a small secreted effector implicated in bacterial persistence, contributes to S. aureus membrane architecture and fluidity. Interestingly, isogenic mutants lacking EsxC, T7SS effectors (EsxA or EsxB), and the membrane-bound EssC are more sensitive to killing by the host-specific fatty acid, linoleic acid (LA), compared to the wild-type. We demonstrate that LA induces more cell membrane damage in the T7SS mutants, although they do not bind differentially to LA. Membrane lipid profiles show that T7SS mutants are also less able to incorporate LA into their membrane phospholipids. Proteomics analyses of wild-type and mutant cell fractions reveal that, in addition to compromising membranes, T7SS defects readily induce bacterial stress and hamper their response to LA challenge. Together, our findings indicate that T7SS is crucial for S. aureus membrane integrity and homeostasis, which is critical when bacteria encounter antimicrobial fatty acids.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_10:09:13.013.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013081
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	Ammonia-loss; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-13 04:17:41	ID requested
⏵ 1	2021-09-08 10:09:13	announced

Kengmo Tchoupa A, Watkins KE, Jones RA, Kuroki A, Alam MT, Perrier S, Chen Y, Unnikrishnan M, The type VII secretion system protects Staphylococcus aureus against antimicrobial host fatty acids. Sci Rep, 10(1):14838(2020) [pubmed]

submitter keyword: Staphylococcus aureus, Type VII secretion system, long-chain unsaturated free fatty acids

Meera Unnikrishnan
contact affiliation	Microbiology and Infection Group, Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, United Kingdom
contact email	M.Unnikrishnan@warwick.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013081
     1. Label: PRIDE project
     2. Name: The type VII secretion system protects Staphylococcus aureus against host-specific fatty acids