PXD013072

PXD013072 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of FSCN1-Interacting Proteins in LSCC cells
Description	Fascin actin-bundling protein 1 (FSCN1) is an evolutionarily conserved actin-bundling protein that plays a critical role in cell migration, motility, adhesion and cellular interactions. Although multiple clinical studies have implicated the expression of FSCN1 in laryngeal squamous cell carcinoma (LSCC) progression, the precise mechanism of FSCN1 in the process has not been clearly elucidated. To define FSCN1 function, we characterized FSCN1-interacting proteins in LSCC cells by immunoprecipitation followed by LC-MS/MS. After data filtering, 119 proteins with expression in both the Hep-2 and TU-177 cell samples were identified as FSCN1-interacting partners. With in-depth bioinformatics analysis, we linked FSCN1 to critical cellular processes including cell adhesion, glycolysis/gluconeogenesis, regulation of protein ubiquitination, ribosomal RNA processing and small molecule metabolism. We discuss the interactions between FSCN1 and some of the newly validated partners. The identification of these potential partners of FSCN1 expands our knowledge of the FSCN1 interactome and provides a valuable resource for understanding the functions of this protein in LSCC progression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:45.876.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liu Hongliang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-13 03:45:54	ID requested
1	2019-09-09 09:28:06	announced
⏵ 2	2024-10-22 04:10:54	announced	2024-10-22: Updated project metadata.

10.1002/iub.2121;

Liu H, Cui J, Zhang Y, Niu M, Xue X, Yin H, Tang Y, Dai L, Dai F, Guo Y, Wu Y, Gao W, Mass spectrometry-based proteomic analysis of FSCN1-interacting proteins in laryngeal squamous cell carcinoma cells. IUBMB Life, 71(11):1771-1784(2019) [pubmed]

submitter keyword: LSCC, LS-MS/MS,FSCN1, interactomics, immunoprecipitation

Wang Binquan
contact affiliation	Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University,China
contact email	wbq_xy@sxent.org
lab head
Liu Hongliang
contact affiliation	Shanxi Medical University
contact email	liuhl2018@sxent.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013072

PRIDE project URI

• PRIDE
  1. PXD013072
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of FSCN1-Interacting Proteins in LSCC cells