PXD013071

PXD013071 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based MS analysis of nasopharyngeal carcinoma cells with inflammasome activation
Description	We previously reported that tumor-cell inflammasomes play a key role in tumor control and act as favorable prognostic markers in nasopharyngeal carcinoma (NPC). Activated inflammasomes frequently form distinguishable ASC specks and govern the cellular secretion of IL-1. However, we know little about the biological and biochemical differences between cells with and without ASC speck formation. In this study, we used proteomic iTRAQ analysis to analyze the proteomes of NPC cells that differ in their apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) speck formation upon cisplatin treatment. We identified proteins that were differentially over-expressed in cells with specks, and found that they fell into two Gene ontology (GO) pathways: mitochondrial oxidative phosphorylation (OxPhos) and ubiquinone metabolism. We observed up-regulation of various components of the OxPhos machinery (including NDUFB3, NDUFB8 and ATP5B), and subsequently found that these changes lead to mitochondrial ROS (mtROS) production, which promotes the formation and activation of NLRP3 inflammasomes and subsequent pyroptosis. In NPC patients with [Define this term?] high-NLRP3 tumors, better recurrence-free survival was significantly associated with high-level expression of NDUFB8 (P=0.037) and ATP5B (P=0.029), as examined using immunohistochemistry. Together, our results demonstrate that upregulated mitochondrial OxPhos components are strongly associated with NLRP3 inflammasome activation in NPC. Our findings further suggest that high-level expression of OxPhos components could be prognostic markers and/or promising therapeutic targets in patients with NPC.
HostingRepository	PRIDE
AnnounceDate	2019-11-15
AnnouncementXML	Submission_2019-11-15_01:22:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chih-Ching Wu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-13 03:43:29	ID requested
1	2019-11-15 01:09:04	announced
⏵ 2	2019-11-15 01:22:36	announced	2019-11-15: Updated publication reference for PubMed record(s): 31723016.

Chung IC, Chen LC, Tsang NM, Chuang WY, Liao TC, Yuan SN, OuYang CN, Ojcius DM, Wu CC, Chang YS, Mitochondrial Oxidative Phosphorylation Complex Regulates NLRP3 Inflammasome Activation and Predicts Patient Survival in Nasopharyngeal Carcinoma. Mol Cell Proteomics, 19(1):142-154(2020) [pubmed]

submitter keyword: iTRAQ, inflammasome, nasopharyngeal carcinoma, mitochondrial oxidative phosphorylation

Chih-Ching Wu
contact affiliation	Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan
contact email	luckywu@mail.cgu.edu.tw
lab head
Chih-Ching Wu
contact affiliation	Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University
contact email	luckywu@mail.cgu.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013071

PRIDE project URI

• PRIDE
  1. PXD013071
     1. Label: PRIDE project
     2. Name: iTRAQ-based MS analysis of nasopharyngeal carcinoma cells with inflammasome activation