PXD013065

PXD013065 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteomic and physiological analyses provide insights into the formation of the variegated leaf in Catalpa fargesii
Description	To unveil the role of posttranslational modification in the variegated phenotype, we conducted global quantitative phosphoproteomic analysis on different leaf color sectors of Maiyuanjinqiu and the corresponding of Catalpa fargesii using Ti4+-IMAC phosphopeptide enrichment. 3778 phosphorylated sites assigned to 1646 phosphoproteins were identified, and 3221 in 1434 proteins were quantified. Differential phosphoproteins (above 1.5 or below 1/1.5) in various leaf color sectors were selected for functional enrichment analyses. GO enrichment revealed that processes of photosynthesis, regulation of the generation of precursor metabolites, response to stress, homeostasis, amino acid metabolism, transport–related processes and most of the energy metabolisms might contribute to leaf color. KEGG pathway enrichment analysis was performed based on differential phosphoproteins (DPs) in different organelles. The result showed that most enriched pathways were located in the chloroplasts and cytosol. The phosphorylation levels of glycometabolism enzymes might greatly affect leaf variegation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:56.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nan Wang
SpeciesList	scientific name: Catalpa fargesii; NCBI TaxID: 71605;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-12 08:58:06	ID requested
1	2019-11-11 16:55:33	announced
⏵ 2	2024-10-22 04:10:57	announced	2024-10-22: Updated project metadata.

10.3390/ijms20081895;

Wang N, Zhu T, Lu N, Wang Z, Yang G, Qu G, Kong L, Zhang S, Ma W, Wang J, . Int J Mol Sci, 20(8):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Catalpa fargesii

phosphoproteomics

variegated leaf

regulated mechanism

Junhui Wang
contact affiliation	State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry
contact email	wangjh@caf.ac.cn
lab head
Nan Wang
contact affiliation	Chinese Academy of Forestry
contact email	wwangnan@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD013065

PRIDE project URI

• PRIDE
  1. PXD013065
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteomic and physiological analyses provide insights into the formation of the variegated leaf in Catalpa fargesii