PXD013058

PXD013058 is an original dataset announced via ProteomeXchange.

Title	CORRELATION OF CANINE BONE MARROW DERIVED MESENCHYMAL STEM CELLS GENOMICS WITH SECRETOME PROTEOMIC COMPOSITION
Description	The adult stem cells have become prominent candidates for the treatment of various diseases in veterinary practice. Therefore, the main goal of our study was to provide a complex study of canine bone marrow derived mesenchymal stem cells (BMMSCs) and conditioned media, isolated from healthy adult dogs of different breeds. Under well-defined standardized isolation protocols the multilineage differentiation and specific surface markers of MSCs were supplemented with their gene expression and the proteome profile. Present data has confirmed that canine BMMSCs expresses important genes for differentiation toward osteo-, chondro- and tendo-genic directions, but also genes that were associated with neurotrophic and immunomodulatory properties. Furthermore, we have identified for the first time by proteome profiling dynamic release various bioactive molecules, such as transcription and translation factors, osteogenic, growth and neurotrophic factors from canine BMMSC conditioned medium. Importantly, relevant genes were linked to their proteins that were detected in conditioned medium, which have an important correlation to their possible function and clinical application. Thus, here we show that the canine BMMSCs properties are applicable for replacement therapies of skeletal disease, but due to their ability of releasing immunomodulatory and bioactive molecules via conditioned media, they may improve repair of other tissue. However, extensive experimental or pre-clinical trials testing canine sources needs to be performed in order to better understand the individual differences between healthy donors that are considered for transplantation treatment studies in veterinary medicine.
HostingRepository	PRIDE
AnnounceDate	2019-07-15
AnnouncementXML	Submission_2019-07-15_03:54:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soulaimane Aboulouard
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-12 03:46:54	ID requested
⏵ 1	2019-07-15 03:54:11	announced

Humenik F, Cizkova D, Cikos S, Luptakova L, Madari A, Mudronova D, Kuricova M, Farbakova J, Spirkova A, Petrovova E, Cente M, Mojzisova Z, Aboulouard S, Murgoci AN, Fournier I, Salzet M, Canine Bone Marrow-derived Mesenchymal Stem Cells: Genomics, Proteomics and Functional Analyses of Paracrine Factors. Mol Cell Proteomics, 18(9):1824-1835(2019) [pubmed]

submitter keyword: Canine, LC-MSMS, Proteomic, stem-cell, secretome

Michel Salzet
contact affiliation	PRISM - Unité INSERM 1192 Université de Lille France
contact email	michel.salzet@univ-lille.fr
lab head
Soulaimane Aboulouard
contact affiliation	PRISM
contact email	soulaimane.aboulouard@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013058

PRIDE project URI

• PRIDE
  1. PXD013058
     1. Label: PRIDE project
     2. Name: CORRELATION OF CANINE BONE MARROW DERIVED MESENCHYMAL STEM CELLS GENOMICS WITH SECRETOME PROTEOMIC COMPOSITION