PXD013055

PXD013055 is an original dataset announced via ProteomeXchange.

Title	The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion
Description	Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events downstream of these receptors have been challenging to resolve. We report here that the homophilic receptor PTPRK is stabilized at cell-cell contacts in epithelial cells. By combining interaction studies, quantitative tyrosine phosphoproteomics, proximity labelling and dephosphorylation assays we identify high confidence PTPRK substrates. PTPRK directly and selectively dephosphorylates at least five substrates, including Afadin, PARD3 and -catenin family members, which are all important cellcell adhesion regulators. In line with this, loss of PTPRK phosphatase activity leads to disrupted cell junctions and increased invasive characteristics. Thus, identifying PTPRK substrates provides insight into its downstream signaling and a potential molecular explanation for its proposed tumor suppressor function.
HostingRepository	PRIDE
AnnounceDate	2019-03-29
AnnouncementXML	Submission_2019-04-02_04:13:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack Houghton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-12 02:49:12	ID requested
1	2019-03-29 11:24:22	announced
⏵ 2	2019-04-02 04:13:13	announced	Updated publication reference for PubMed record(s): 30924770.

Fearnley GW, Young KA, Edgar JR, Antrobus R, Hay IM, Liang WC, Martinez-Martin N, Lin W, Deane JE, Sharpe HJ, The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion. Elife, 8():(2019) [pubmed]

curator keyword: Biological

submitter keyword: Phosphorylation

SILAC

Dr Hayley J Sharpe
contact affiliation	Cambridge Institute for Medical Research
contact email	hjs49@cam.ac.uk
lab head
Jack Houghton
contact affiliation	University of Cambridge
contact email	jwh65@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD013055

PRIDE project URI

• PRIDE
  1. PXD013055
     1. Label: PRIDE project
     2. Name: The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion