PXD013051

PXD013051 is an original dataset announced via ProteomeXchange.

Title	Eukaryotic Ribosome GCN2 interaction Hydrogen/Deuterium Exchange Mass Spectrometry
Description	General control nonderepressible 2 (GCN2) phosphorylates eIF2α, regulating translation in response to nutritional stress. Here, we show that mammalian ribosomes are potent GCN2 activators. Hydrogen/deuterium exchange–mass spectrometry (HDX-MS) showed GCN2 interacting with domain II of the uL10 P-stalk protein. The P-stalk is a uL10/P12/P22 pentameric complex that is part of the ribosomal GTPase-associated center. Recombinant human P-stalk greatly stimulates GCN2. Both domain II of uL10 and the C-terminal tails of P1 and P2 are necessary for maximal GCN2 activation. On actively translating ribosomes, the C-terminal tails of P1 and P2 are sequestered by elongation factors, suggesting P-stalk availability could link translational stress to GCN2 activation.
HostingRepository	PRIDE
AnnounceDate	2019-05-24
AnnouncementXML	Submission_2019-05-24_07:20:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Glenn Masson
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationList	phosphorylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-12 02:48:20	ID requested
⏵ 1	2019-05-24 07:20:47	announced

Inglis AJ, Masson GR, Shao S, Perisic O, McLaughlin SH, Hegde RS, Williams RL, Activation of GCN2 by the ribosomal P-stalk. Proc Natl Acad Sci U S A, 116(11):4946-4954(2019) [pubmed]

curator keyword: Biological

submitter keyword: Ribosome, GCN2, HDX-MS

Roger L. Williams
contact affiliation	MRC Laboratory of Molecular Biology
contact email	rlw@mrc-lmb.cam.ac.uk
lab head
Glenn Masson
contact affiliation	MRC Laboratory of Molecular Biology
contact email	gmasson@mrc-lmb.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013051
     1. Label: PRIDE project
     2. Name: Eukaryotic Ribosome GCN2 interaction Hydrogen/Deuterium Exchange Mass Spectrometry