PXD013034

PXD013034 is an original dataset announced via ProteomeXchange.

Title	Epstein-Barr Virus Induced One-Carbon Metabolism Drives B-Cell Transformation
Description	Epstein-Barr virus (EBV) causes Burkitt, Hodgkin, and post-transplant B-cell lymphomas. How EBV remodels metabolic pathways to support rapid B-cell outgrowth remains largely unknown. To gain insights, primary human B-cells were profiled by tandem-mass-tag based proteomics at rest and at 9 time points after infection. >8000 host and 29 viral proteins were quantified, revealing mitochondrial remodeling and induction of one-carbon (1C) metabolism. EBV-encoded EBNA2 and its target MYC were required for upregulation of the central mitochondrial 1C enzyme MTHFD2, which played key roles in EBV-driven B-cell growth and survival. MTHFD2 was critical for maintaining elevated NADPH levels in infected cells, and oxidation of mitochondrial NADPH diminished B-cell proliferation. Tracing studies underscored contributions of 1C to nucleotide synthesis, NADPH production and redox defense. EBV upregulated import and synthesis of serine to augment 1C flux. Our results highlight EBV-induced 1C as a potential therapeutic target and provide a new paradigm for viral onco-metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:11:20.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Weekes
SpeciesList	scientific name: Epstein-barr virus strain p3hr-1; NCBI TaxID: 82829; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; carbamoylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-11 02:40:22	ID requested
1	2019-06-28 03:16:17	announced
2	2019-07-04 04:57:45	announced	Updated publication reference for PubMed record(s): 31257153.
⏵ 3	2024-10-22 04:11:24	announced	2024-10-22: Updated project metadata.

10.1016/j.cmet.2019.06.003;

Wang LW, Shen H, Nobre L, Ersing I, Paulo JA, Trudeau S, Wang Z, Smith NA, Ma Y, Reinstadler B, Nomburg J, Sommermann T, Cahir-McFarland E, Gygi SP, Mootha VK, Weekes MP, Gewurz BE, Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation. Cell Metab, 30(3):539-555.e11(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: herpesvirus, one-carbon metabolism,Epstein-Barr virus, host-pathogen interaction, quantitative proteomics, tandem mass tag

Michael Weekes
contact affiliation	Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 0XY
contact email	Mpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliation	University of Cambridge
contact email	mpw1001@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013034

PRIDE project URI

• PRIDE
  1. PXD013034
     1. Label: PRIDE project
     2. Name: Epstein-Barr Virus Induced One-Carbon Metabolism Drives B-Cell Transformation