PXD013016

PXD013016 is an original dataset announced via ProteomeXchange.

Title	Interactions between Transport Protein Particle (TRAPP) complexes and Rab GTPases in Arabidopsis
Description	The Transport Protein Particle II (TRAPPII) is essential for exocytosis, endocytosis, protein sorting and cytokinesis. In spite of a considerable understanding of its biological role, little is known about Arabidopsis TRAPPII complex topology and molecular function. In this study, independent proteomic approaches initiated with TRAPP components or Rab-A GTPase variants converge on the TRAPPII complex. We show that the Arabidopsis genome encodes the full complement of 13 TRAPPC subunits, including four previously unidentified components. A dimerization model is proposed to account for binary interactions between TRAPPII subunits. Preferential binding to dominant negative (GDP-bound) versus wild-type or constitutively active (GTP-bound) RAB-A2a variants discriminates between TRAPPII and TRAPPIII subunits and shows that Arabidopsis complexes differ from yeast but resemble metazoan TRAPP complexes. While the TRAPPII complex has been implicated in biosynthetic pathways, TRAPPIII appears to be implicated in degradative pathways. Analyses of Rab-A variants in trappii backgrounds provide genetic evidence for the conclusion that the TRAPPII complex behaves as a Guanine-nucleotide Exchange Factor (GEF) for the RAB-A2a GTPase. GEFs catalyze exchange of GDP for GTP; the GTP-bound, activated, Rab then recruits a diverse local network of Rab effectors to specify membrane identity in subsequent vesicle fusion events. Understanding the control of GEF-Rab interactions will be crucial to unraveling the co-ordination of membrane traffic.
HostingRepository	PRIDE
AnnounceDate	2019-09-03
AnnouncementXML	Submission_2019-09-03_09:01:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-08 07:19:12	ID requested
⏵ 1	2019-09-03 09:01:10	announced

Kalde M, Elliott L, Ravikumar R, Rybak K, Altmann M, Klaeger S, Wiese C, Abele M, Al B, Kalbfu, ß N, Qi X, Steiner A, Meng C, Zheng H, Kuster B, Falter-Braun P, Ludwig C, Moore I, Assaad FF, Interactions between Transport Protein Particle (TRAPP) complexes and Rab GTPases in Arabidopsis. Plant J, 100(2):279-297(2019) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, Rab GTPases, Co-IP, Transport Protein Particle, TRAPPII, protein complex

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry Technische Universität München Gregor-Mendel-Straße 4 85354 Freising DEUTSCHLAND / GERMANY
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013016

PRIDE project URI

• PRIDE
  1. PXD013016
     1. Label: PRIDE project
     2. Name: Interactions between Transport Protein Particle (TRAPP) complexes and Rab GTPases in Arabidopsis