PXD012997

PXD012997 is an original dataset announced via ProteomeXchange.

Title	Isolation of repaired chromatin of UVC-irradiated human skin fibroblasts
Description	To further study and understand the DNA damage responses, we developed aniFOUND, a new method for capturing under native conditions the repaired DNA and the proteins associated with it after UVC irradiation. hTert-immortalised human skin fibroblasts were irradiated with 20 J/m2 UVC and the nascent DNA that resulted from the Unscheduled DNA Synthesis was specifically labeled with nucleotide analogues (EdU). This newly repaired/synthesized DNA together with the bound proteins were pulled-down with streptavidin beads. For negative controls we used both irradiated, but non-labelled samples (+UV/-EdU) and labelled, but not irradiated cells (-UV/+EdU). The pulldowns were eluted from the beads by boiling in 0.1 % SDS.
HostingRepository	PRIDE
AnnounceDate	2021-03-10
AnnouncementXML	Submission_2021-03-09_23:17:56.162.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-06 07:12:11	ID requested
⏵ 1	2021-03-09 23:17:56	announced

Dataset with its publication pending

submitter keyword: aniFOUND, UV-C radiation, chromatin, DNA repair, DNA damage responses, Nucleotide excision repair-NER, human fibroblasts, mass spectrometry

Maria Fousteri
contact affiliation	Biomedical Sciences Research Center 'Alexander Fleming' 34 Fleming St., 16672, Vari, Greece.
contact email	fousteri@fleming.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD012997

PRIDE project URI

• PRIDE
  1. PXD012997
     1. Label: PRIDE project
     2. Name: Isolation of repaired chromatin of UVC-irradiated human skin fibroblasts