PXD012992

PXD012992 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of LDIP mutant phenotypes in Arabidopsis seedlings
Description	Lipid droplets (LDs) are unique, protein-coated organelles found in all eukaryotic organisms that function primarily in the storage of energy-rich neutral lipids, such as triacylglycerols (TAGs). In plants, LDs are found in high abundance in oilseeds, and proteins involved in their formation and activity, such as oleosins, have been well-characterized. Our understanding of LD biogenesis and activity in other cell types, as well as the proteins that regulate these processes, is not as comprehensive. We recently identified and characterized a novel Arabidopsis LD coat protein termed LDIP (LDAP-interacting protein) that regulates LD size and neutral lipid homeostasis in leaf and seed embryonic cells. Here, we show that LDIP plays a distinct role in regulating LD size during biogenesis in leaf cells via interactions with key biogenetic proteins. Localization experiments performed in both plant and insect cells revealed LDIP is necessarily recruited to the LD surface by LDAP3 and binds nascent LDs during early-stage LD formation. Pull-down and Y2H assays indicate LDIP interacts with the biogenetic proteins SEIPINs, while combinatory expression levels of these proteins resulted in aberrant LD size and number in both plant cells and yeast. Evidence is also provided that LDIP plays a key role in modulating phospholipid levels in both leaves and seeds. Taken together, our data allow for the generation of model for LD biogenesis in non-seed cell types.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_11:07:39.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Till Ischebeck
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-06 02:23:17	ID requested
⏵ 1	2022-02-15 11:07:41	announced

Pyc M, Gidda SK, Seay D, Esnay N, Kretzschmar FK, Cai Y, Doner NM, Greer MS, Hull JJ, Coulon D, Br, é, h, é, lin C, Yurchenko O, de Vries J, Valerius O, Braus GH, Ischebeck T, Chapman KD, Dyer JM, Mullen RT, LDIP cooperates with SEIPIN and LDAP to facilitate lipid droplet biogenesis in Arabidopsis. Plant Cell, 33(9):3076-3103(2021) [pubmed]

curator keyword: Biological

submitter keyword: Lipid droplets, Arabidopsis thaliana, LDAP-interacting protein, LDIP

D. Till Ischebeck
contact affiliation	Biochemie der Pflanze, Justus-von-Liebig-Weg 11, 37077 Göttingen
contact email	tischeb@gwdg.de
lab head
Till Ischebeck
contact affiliation	University of Goettingen
contact email	tischeb@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012992
     1. Label: PRIDE project
     2. Name: Proteomic characterization of LDIP mutant phenotypes in Arabidopsis seedlings