PXD012984

PXD012984 is an original dataset announced via ProteomeXchange.

Title	Cryopreservation differently alters proteome in epididymal and ejaculated pig spermatozoa
Description	Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate with the loss of sperm function during cryopreservation remains unsolved. The present study attempted to clarify this issue evaluating differential changes in the proteome of pig spermatozoa retrieved from the cauda epididymis and the ejaculate, with clear differences in cryotolerance, comparing fresh and frozen-thawed cells. Sperm samples were collected from 10 healthy, sexually mature and fertile boars, and cryopreserved using a standard 0.5 mL straw protocol. Total and progressive motility, viability and mitochondria membrane potential were higher and membrane fluidity and reactive oxygen species generation lower in frozen-thawed (FT) cauda epididymal than ejaculated spermatozoa. Quantitative proteomics of fresh and FT sperm samples were analyzed using a LC-ESI-MS/MS-based SWATH approach. Cryopreservation quantitatively altered more proteins in ejaculated than cauda epididymal spermatozoa. Differential protein-protein networks highlighted a set of proteins directly involved in mitochondrial functionality among those quantitatively altered in ejaculated spermatozoa, which would explain the worse post-thaw quality of ejaculated pig spermatozoa.
HostingRepository	PRIDE
AnnounceDate	2019-11-11
AnnouncementXML	Submission_2019-11-11_14:45:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-05 08:46:59	ID requested
⏵ 1	2019-11-11 14:45:14	announced

Perez-Pati, ñ, o C, Barranco I, Li J, Padilla L, Martinez EA, Rodriguez-Martinez H, Roca J, Parrilla I, Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa. Int J Mol Sci, 20(7):(2019) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: epididymis, ejaculate, spermatozoa, cryopreservation, proteomics, porcinepig

Jordi Roca
contact affiliation	Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain
contact email	roca@um.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012984

PRIDE project URI

• PRIDE
  1. PXD012984
     1. Label: PRIDE project
     2. Name: Cryopreservation differently alters proteome in epididymal and ejaculated pig spermatozoa