PXD012979

PXD012979 is an original dataset announced via ProteomeXchange.

Title	Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells
Description	Diabetes is a global health problem caused primarily by the inability of pancreatic β-cells to secrete adequate levels of insulin. The molecular mechanisms underlying the progressive failure of β-cells to respond to glucose in type-2 diabetes remain unresolved. Using a combination of transcriptomics and proteomics, we find significant dysregulation of major metabolic pathways in islets of diabetic βV59M mice, a non-obese, eulipidaemic diabetes model. Multiple genes/proteins involved in glycolysis/gluconeogenesis are upregulated, whereas those involved in oxidative phosphorylation are downregulated. In isolated islets, glucose-induced increases in NADH and ATP are impaired and both oxidative and glycolytic glucose metabolism are reduced. INS-1 β-cells cultured chronically at high glucose show similar changes in protein expression and reduced glucose-stimulated oxygen consumption: targeted metabolomics reveals impaired metabolism. These data indicate hyperglycaemia induces metabolic changes in β-cells that markedly reduce mitochondrial metabolism and ATP synthesis. We propose this underlies the progressive failure of β-cells in diabetes.
HostingRepository	PRIDE
AnnounceDate	2019-04-10
AnnouncementXML	Submission_2019-06-20_03:35:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012979
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Roman Fischer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Oxidation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-05 07:29:03	ID requested
1	2019-04-10 12:36:26	announced
⏵ 2	2019-06-20 03:35:54	announced	Updated publication reference for PubMed record(s): 31171772.

Haythorne E, Rohm M, van de Bunt M, Brereton MF, Tarasov AI, Blacker TS, Sachse G, Silva Dos Santos M, Terron Exposito R, Davis S, Baba O, Fischer R, Duchen MR, Rorsman P, MacRae JI, Ashcroft FM, -cells. Nat Commun, 10(1):2474(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: diabetes, β-cells, islets, pancreas

Roman Fischer
contact affiliation	Discovery Proteomics Facility University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
lab head
Roman Fischer
contact affiliation	University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012979

PRIDE project URI

• PRIDE
  1. PXD012979
     1. Label: PRIDE project
     2. Name: Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells