PXD012977

PXD012977 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of the oncogenic septin 9 reveals isoform-specific interactions in breast cancer cells
Description	Septins are a family of multimeric GTP-binding proteins, which are abnormally expressed in cancer. Septin 9 (SEPT9) is an essential and ubiquitously expressed septin with multiple isoforms, which have differential expression patterns and effects in breast cancer cells. It is unknown, however, if SEPT9 isoforms associate with different molecular networks and functions. Here, we performed a proteomic screen in MCF-7 breast cancer cells to identify the interactome of GFP-SEPT9 isoforms 1, 4 and 5, which vary in the length of their N-terminus. While all three isoforms associated with SEPT2 and SEPT7, the truncated SEPT9_i4 and SEPT9_i5 interacted with septins of the SEPT6 group more promiscuously than SEPT9_i1, which bound predominately SEPT8. Spatial mapping and functional clustering of non-septin partners showed isoform-specific differences in interactions with proteins of distinct subcellular organelles (nuclei, centrosomes, cilia) and functions (signaling, degradation). Notably, the interactome of the full length SEPT9_i1 was more enriched in cytoskeletal regulators, while the truncated SEPT9_i4 and SEPT9_i5 exhibited preferential and isoform-specific interactions with nuclear and signaling factors as well as ubiquitinating enzymes. These data provide evidence for isoform-specific interactions, which arise from truncations in the N-terminal extensions of SEPT9, and point to novel roles in the pathogenesis of breast cancer.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:20:35.095.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012977
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Louis Devlin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; Carbamidomethyl
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-05 07:26:22	ID requested
1	2022-10-13 04:43:02	announced
⏵ 2	2023-11-14 07:20:38	announced	2023-11-14: Updated project metadata.

Devlin L, Okletey J, Perkins G, Bowen JR, Nakos K, Montagna C, Spiliotis ET, Proteomic profiling of the oncogenic septin 9 reveals isoform-specific interactions in breast cancer cells. Proteomics, 21(19):e2100155(2021) [pubmed]

10.6019/PXD012977;

submitter keyword: breast cancer, septin interactome,septins, SEPT9

ELIAS T SPILIOTIS, PHD
contact affiliation	Drexel University College of Arts and Sciences, Departmentof Biology, United States
contact email	ets33@drexel.edu
lab head
Louis Devlin
contact affiliation	Drexel University
contact email	devlin.louis@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD012977

PRIDE project URI

• PRIDE
  1. PXD012977
     1. Label: PRIDE project
     2. Name: Proteomic profiling of the oncogenic septin 9 reveals isoform-specific interactions in breast cancer cells