PXD012967

PXD012967 is an original dataset announced via ProteomeXchange.

Title	Bacteriocins in Lactobacillus acidophlius membrane vesicles
Description	Recent reports have shown that Gram-positive bacteria actively secrete spherical nanometer-sized proteolipid membrane vesicles (MVs) into their surroundings. Though MVs have been implicated in a broad range of biological functions, few studies have been conducted to examine their potential as delivery vehicles of antimicrobials. Here, we investigate the natural ability of Lactobacillus acidophilus MVs to carry and deliver bacteriocin peptides to the opportunistic pathogen, Lactobacillus delbrueckii. We demonstrate that upon treatment with lactacin B-inducing peptide the proteome of the secreted MVs is enriched in putative bacteriocins encoded by the lab operon. Further, we show that purified MVs inhibit growth and form membrane pores in L. delbrueckii, which is confirmed using a number of microscopy techniques and spectrophotometry. These results show that L. acidophilus MVs serve as conduits for antimicrobials to competing cells in the environment, suggesting a potential role for MVs in complex communities such as the gut microbiome. With the potential for controlling their payload through microbial engineering, MVs produced by L. acidophilus may be an interesting platform for effecting change in complex microbial communities or aiding in the development of new biomedical therapeutics.
HostingRepository	PRIDE
AnnounceDate	2020-03-20
AnnouncementXML	Submission_2020-06-12_03:49:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012967
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Scott Dean
SpeciesList	scientific name: Lactobacillus acidophilus NCFM; NCBI TaxID: 272621;
ModificationList	Deamidated; Oxidation
Instrument	TripleTOF 5600; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-05 03:56:49	ID requested
1	2020-03-20 00:44:19	announced
⏵ 2	2020-06-12 03:49:27	announced	2020-06-12: Updated publication reference for PubMed record(s): 32425905.

Dean SN, Rimmer MA, Turner KB, Phillips DA, Caruana JC, Hervey WJ, Leary DH, Walper SA, Membrane Vesicles as a Vehicle of Bacteriocin Delivery. Front Microbiol, 11():710(2020) [pubmed]

curator keyword: Biological

submitter keyword: Lactobacillus, membrane vesicles, targeted proteomics

Scott Walper
contact affiliation	Naval Research Laboratory
contact email	scott.walper@nrl.navy.mil
lab head
Scott Dean
contact affiliation	Naval Research Laboratory
contact email	scottnathandean@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012967
     1. Label: PRIDE project
     2. Name: Bacteriocins in Lactobacillus acidophlius membrane vesicles