PXD012957

PXD012957 is an original dataset announced via ProteomeXchange.

Title	Grb2 interactome data with and without EGF stimulation
Description	Growth factor receptor-bound protein 2 or Grb2 is an adaptor protein involved in signal transduction. It is widely expressed and integral to several cellular functions, including cell proliferation. We designed this study to capture interacting partners of Grb2 in absence and presence of epidermal growth factor (EGF). HEK 293 cells, expressing tagged Grb2, were cultured with SILAC labels in presence and absence of EGF. Grb2 interactome was enriched by immunoprecipitation and subjected to mass spectrometry. This data will help in understanding the regulatory effect of EGF stimulation on Grb2 protein and its interactors.
HostingRepository	PRIDE
AnnounceDate	2019-03-06
AnnouncementXML	Submission_2019-07-04_04:48:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ajay Kumar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-05 01:55:54	ID requested
1	2019-03-05 04:19:31	announced
2	2019-03-06 00:30:17	announced	Updated project metadata.
⏵ 3	2019-07-04 04:48:33	announced	Updated project metadata.

Duggal S, Midha MK, Kumar A, Rao KVS, Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor. Data Brief, 25():104082(2019) [pubmed]

curator keyword: Biological

submitter keyword: Grb2, EGF, Interactome, SILAC, AP-MS

Ajay Kumar
contact affiliation	Drug Discovery Research Center Translational Health Science and Technology Institute NCR Biotech Science Cluster Faridabad-121001. Haryana. India
contact email	a.kumar@thsti.res.in
lab head
Ajay Kumar
contact affiliation	THSTI, Faridabad
contact email	ajay22k@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012957

PRIDE project URI

• PRIDE
  1. PXD012957
     1. Label: PRIDE project
     2. Name: Grb2 interactome data with and without EGF stimulation