PXD012951

PXD012951 is an original dataset announced via ProteomeXchange.

Title	NET induced by different stimuli: a comparative proteomic analysis
Description	Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger the production of autoantibodies and cause organ damage in autoimmune disorders. Spontaneously netting neutrophils are not frequent and induction of NET in vitro by selected stimuli is necessary to investigate their structure. In the present work, the protein composition and post-translational modifications of NET produced under different stimuli is studied by means of proteomic analysis. Neutrophils from healthy donors were stimulated by PMA, A23187, E.Coli LPS or untreated; after 3 hours cells were washed, treated with DNase and supernatants collected for mass spectrometry. Data were analyzed by unsupervised hierarchical clustering analyses. We identified proteins contained in NETs of any source or exclusive of one stimulus: LPS-induced and spontaneous NET diverge in protein composition, while PMA- and A23187-induced NET appear more similar. Among the post-translational modifications we examined, methionine sulfoxidation is frequent especially in PMA- and LPS-induced NETs. Myeloperoxidase is the protein more extensively modified. Thus, proteomic analysis indicates that NETs induced by different stimuli are heterogeneous in terms of both protein composition and post-translational modifications, suggesting that NET induced in different conditions may have different biological effects.
HostingRepository	PRIDE
AnnounceDate	2019-07-15
AnnouncementXML	Submission_2019-07-15_02:20:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Petretto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-04 06:02:48	ID requested
⏵ 1	2019-07-15 02:20:48	announced

Petretto A, Bruschi M, Pratesi F, Croia C, Candiano G, Ghiggeri G, Migliorini P, Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis. PLoS One, 14(7):e0218946(2019) [pubmed]

submitter keyword: neutrophil extracellular traps

proteomics

post-translational modifications

Andrea Petretto
contact affiliation	Ist. G. Gaslini
contact email	andreapetretto@gaslini.org
lab head
Andrea Petretto
contact affiliation	Gaslini Children's Hospital
contact email	a.petretto@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012951
     1. Label: PRIDE project
     2. Name: NET induced by different stimuli: a comparative proteomic analysis